3i17

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{{Seed}}
 
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[[Image:3i17.png|left|200px]]
 
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==Crystal structure of the apo R132K:L121E mutant of cellular retinoic acid-binding protein II at 1.68 angstrom resolution==
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The line below this paragraph, containing "STRUCTURE_3i17", creates the "Structure Box" on the page.
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<StructureSection load='3i17' size='340' side='right'caption='[[3i17]], [[Resolution|resolution]] 1.68&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3i17]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I17 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I17 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.68&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i17 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i17 OCA], [https://pdbe.org/3i17 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i17 RCSB], [https://www.ebi.ac.uk/pdbsum/3i17 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i17 ProSAT]</span></td></tr>
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{{STRUCTURE_3i17| PDB=3i17 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RABP2_HUMAN RABP2_HUMAN] Transports retinoic acid to the nucleus. Regulates the access of retinoic acid to the nuclear retinoic acid receptors.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i1/3i17_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3i17 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Cellular Retinoic Acid Binding Protein II (CRABPII) has been reengineered to specifically bind and react with all-trans-retinal to form a protonated Schiff base. Each step of this process has been dissected and four residues (Lys132, Tyr134, Arg111, and Glu121) within the CRABPII binding site have been identified as crucial for imine formation and/or protonation. The precise role of each residue has been examined through site directed mutagenesis and crystallographic studies. The crystal structure of the R132K:L121E-CRABPII (PDB-3I17) double mutant suggests a direct interaction between engineered Glu121 and the native Arg111, which is critical for both Schiff base formation and protonation. Proteins 2009. (c) 2009 Wiley-Liss, Inc.
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===Crystal structure of the apo R132K:L121E mutant of cellular retinoic acid-binding protein II at 1.68 anstrom resolution===
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Elucidating the exact role of engineered CRABPII residues for the formation of a retinal protonated Schiff base.,Vasileiou C, Wang W, Jia X, Lee KS, Watson CT, Geiger JH, Borhan B Proteins. 2009 Jun 8. PMID:19603486<ref>PMID:19603486</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3i17" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19603486}}, adds the Publication Abstract to the page
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*[[CRABP I ( Cellular Retinoic Acid Binding Protein )|CRABP I ( Cellular Retinoic Acid Binding Protein )]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19603486 is the PubMed ID number.
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*[[Cellular retinoic acid-binding protein 3D structures|Cellular retinoic acid-binding protein 3D structures]]
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*[[Gustavo Elberto Epalza Sanchez/Sandbox 1|Gustavo Elberto Epalza Sanchez/Sandbox 1]]
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{{ABSTRACT_PUBMED_19603486}}
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*[[Molecular Playground/CRABP I (Cellular Retinoic Acid Binding Protein)|Molecular Playground/CRABP I (Cellular Retinoic Acid Binding Protein)]]
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== References ==
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==About this Structure==
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<references/>
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3I17 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I17 OCA].
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__TOC__
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:19603486</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Geiger, J H.]]
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[[Category: Large Structures]]
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[[Category: Jia, X.]]
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[[Category: Geiger JH]]
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[[Category: Watson, C T.]]
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[[Category: Jia X]]
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[[Category: Crabpii]]
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[[Category: Watson CT]]
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[[Category: Cytoplasm]]
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[[Category: Lipid binding]]
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[[Category: Nucleus]]
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[[Category: Retinal binding]]
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[[Category: Retinoic acid]]
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[[Category: Retinoid]]
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[[Category: Retinol-binding]]
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[[Category: Transport]]
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[[Category: Vitamin some]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 11 22:56:50 2009''
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Current revision

Crystal structure of the apo R132K:L121E mutant of cellular retinoic acid-binding protein II at 1.68 angstrom resolution

PDB ID 3i17

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