1y0q

[[Image:1y0q.gif|left|200px]]<br /><applet load="1y0q" size="450" color="white" frame="true" align="right" spinBox="true"

caption="1y0q, resolution 3.60&Aring;" />

'''Crystal structure of an active group I ribozyme-product complex'''<br />

==Overview==

Group I introns are catalytic RNAs capable of orchestrating two sequential, phosphotransesterification reactions that result in self-splicing. To, understand how the group I intron active site facilitates catalysis, we, have solved the structure of an active ribozyme derived from the orf142-I2, intron from phage Twort bound to a four-nucleotide product RNA at a, resolution of 3.6 A. In addition to the three conserved domains, characteristic of all group I introns, the Twort ribozyme has peripheral, insertions characteristic of phage introns. These elements form a ring, that completely envelops the active site, where a snug pocket for, guanosine is formed by a series of stacked base triples. The structure of, the active site reveals three potential binding sites for catalytic, metals, and invokes a role for the 2' hydroxyl of the guanosine substrate, in organization of the active site for catalysis.

==About this Structure==

1Y0Q is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Staphylococcus_phage_twort Staphylococcus phage twort] with SPK, MG and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Y0Q OCA].

 

Crystal structure of a phage Twort group I ribozyme-product complex., Golden BL, Kim H, Chase E, Nat Struct Mol Biol. 2005 Jan;12(1):82-9. Epub 2004 Dec 5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15580277 15580277]

[[Category: Protein complex]]

[[Category: Staphylococcus phage twort]]

[[Category: Chase, E.]]

[[Category: Golden, B.L.]]

[[Category: Kim, H.]]