1dnh

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(New page: 200px<br /><applet load="1dnh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dnh, resolution 2.250&Aring;" /> '''THE MOLECULAR STRUC...)
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[[Image:1dnh.jpg|left|200px]]<br /><applet load="1dnh" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1dnh, resolution 2.250&Aring;" />
 
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'''THE MOLECULAR STRUCTURE OF THE COMPLEX OF HOECHST 33258 AND THE DNA DODECAMER D(CGCGAATTCGCG)'''<br />
 
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==Overview==
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==THE MOLECULAR STRUCTURE OF THE COMPLEX OF HOECHST 33258 AND THE DNA DODECAMER D(CGCGAATTCGCG)==
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The crystal structure of the complex between the dodecamer d(CGCGAATTCGCG), and a synthetic dye molecule Hoechst 33258 was solved by X-ray diffraction, analysis and refined to an R-factor of 15.7% at 2.25 A resolution. The, crescent-shaped Hoechst compound is found to bind to the central four AATT, base pairs in the narrow minor groove of the B-DNA double helix. The, piperazine ring of the drug has its flat face almost parallel to the, aromatic bisbenzimidazole ring and lies sideways in the minor groove. No, evidence of disordered structure of the drug is seen in the complex. The, binding of Hoechst to DNA is stabilized by a combination of hydrogen, bonding, van der Waals interaction and electrostatic interactions. The, binding preference for AT base pairs by the drug is the result of the, close contact between the Hoechst molecule and the C2 hydrogen atoms of, adenine. The nature of these contacts precludes the binding of the drug to, G-C base pairs due to the presence of N2 amino groups of guanines. The, present crystal structural information agrees well with the data obtained, from chemical footprinting experiments.
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<StructureSection load='1dnh' size='340' side='right'caption='[[1dnh]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1dnh]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DNH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DNH FirstGlance]. <br>
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1DNH is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with HT as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DNH OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HT:2-(4-HYDROXYPHENYL)-5-(4-METHYL-1-PIPERAZINYL)-2,5-BI-BENZIMIDAZOLE'>HT</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dnh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dnh OCA], [https://pdbe.org/1dnh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dnh RCSB], [https://www.ebi.ac.uk/pdbsum/1dnh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dnh ProSAT]</span></td></tr>
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The molecular structure of the complex of Hoechst 33258 and the DNA dodecamer d(CGCGAATTCGCG)., Teng MK, Usman N, Frederick CA, Wang AH, Nucleic Acids Res. 1988 Mar 25;16(6):2671-90. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2452403 2452403]
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</table>
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[[Category: Protein complex]]
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__TOC__
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[[Category: Frederick, C.A.]]
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</StructureSection>
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[[Category: Teng, M.K.]]
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[[Category: Large Structures]]
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[[Category: Usman, N.]]
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[[Category: Frederick CA]]
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[[Category: Wang, A.H.J.]]
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[[Category: Teng M-K]]
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[[Category: HT]]
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[[Category: Usman N]]
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[[Category: b-dna]]
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[[Category: Wang AH-J]]
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[[Category: complexed with drug]]
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[[Category: double helix]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 03:23:31 2007''
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Current revision

THE MOLECULAR STRUCTURE OF THE COMPLEX OF HOECHST 33258 AND THE DNA DODECAMER D(CGCGAATTCGCG)

PDB ID 1dnh

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