2kg5

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{{Seed}}
 
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[[Image:2kg5.jpg|left|200px]]
 
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==NMR Solution structure of ARAP3-SAM==
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The line below this paragraph, containing "STRUCTURE_2kg5", creates the "Structure Box" on the page.
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<StructureSection load='2kg5' size='340' side='right'caption='[[2kg5]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2kg5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KG5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KG5 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kg5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kg5 OCA], [https://pdbe.org/2kg5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kg5 RCSB], [https://www.ebi.ac.uk/pdbsum/2kg5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kg5 ProSAT]</span></td></tr>
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{{STRUCTURE_2kg5| PDB=2kg5 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ARAP3_HUMAN ARAP3_HUMAN] Phosphatidylinositol 3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. Acts on ARF6, RAC1, RHOA and CDC42. Plays a role in the internalization of anthrax toxin.<ref>PMID:11804589</ref> <ref>PMID:15569923</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kg/2kg5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kg5 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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BACKGROUND: Sterile alpha motif (Sam) domains are small protein modules that can be involved in homotypic or heterotypic associations and exhibit different functions. Previous studies have demonstrated that the Sam domain of the lipid phosphatase Ship2 can hetero-dimerize with the Sam domain of the PI3K effector protein Arap3. RESULTS: Here, we determine the NMR solution structure of Arap3-Sam and implement a multidisciplinary approach consisting of NMR spectroscopy, ITC (Isothermal Titration Calorimetry), mutagenesis and molecular modeling studies to analyze the interaction between Ship2-Sam and Arap3-Sam. This work reveals that Arap3-Sam may associate with Ship2-Sam by adopting a binding mode common to other Sam domains. This binding mode is identical to what we have very recently observed for the association between Ship2-Sam and the Sam domain from the Ephrin A2 receptor. CONCLUSION: Our studies further clarify the structural features that are relevant for Sam-Sam interactions involving Ship2 and give additional hints that could be used for the identification of new molecules able to selectively inhibit Sam-Sam associations.
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===NMR Solution structure of ARAP3-SAM===
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The Sam domain of the lipid phosphatase Ship2 adopts a common model to interact with Arap3-Sam and EphA2-Sam.,Leone M, Cellitti J, Pellecchia M BMC Struct Biol. 2009 Sep 18;9:59. PMID:19765305<ref>PMID:19765305</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19765305}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2kg5" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19765305 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19765305}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2KG5 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KG5 OCA].
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==Reference==
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<ref group="xtra">PMID:19765305</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Leone, M.]]
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[[Category: Large Structures]]
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[[Category: Pellecchia, M.]]
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[[Category: Leone M]]
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[[Category: Cell membrane]]
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[[Category: Pellecchia M]]
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[[Category: Cell projection]]
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[[Category: Cytoplasm]]
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[[Category: Cytoskeleton]]
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[[Category: Gtpase activation]]
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[[Category: Helix bundle]]
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[[Category: Membrane]]
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[[Category: Metal-binding]]
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[[Category: Phosphoprotein]]
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[[Category: Polymorphism]]
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[[Category: Sam domain]]
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[[Category: Signaling protein]]
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[[Category: Zinc]]
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[[Category: Zinc-finger]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 18 19:18:31 2009''
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Current revision

NMR Solution structure of ARAP3-SAM

PDB ID 2kg5

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