1u73

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(New page: 200px<br /><applet load="1u73" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u73, resolution 1.9&Aring;" /> '''Crystal structure of ...)
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[[Image:1u73.gif|left|200px]]<br /><applet load="1u73" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1u73, resolution 1.9&Aring;" />
 
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'''Crystal structure of a Dimeric Acidic Platelet Aggregation Inhibitor and Hypotensive Phospholipase A2 from Bothrops jararacussu'''<br />
 
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==Overview==
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==Crystal structure of a Dimeric Acidic Platelet Aggregation Inhibitor and Hypotensive Phospholipase A2 from Bothrops jararacussu==
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Phospholipases A2 belong to the superfamily of proteins which hydrolyzes, the sn-2 acyl groups of membrane phospholipids to release arachidonic acid, and lysophospholipids. An acidic phospholipase A2 isolated from Bothrops, jararacussu snake venom presents a high catalytic, platelet aggregation, inhibition and hypotensive activities. This protein was crystallized in, two oligomeric states: monomeric and dimeric. The crystal structures were, solved at 1.79 and 1.90 angstroms resolution, respectively, for the two, states. It was identified a Na+ ion at the center of Ca2+-binding site of, the monomeric form. A novel dimeric conformation with the active sites, exposed to the solvent was observed. Conformational states of the molecule, may be due to the physicochemical conditions used in the crystallization, experiments. We suggest dimeric state is one found in vivo.
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<StructureSection load='1u73' size='340' side='right'caption='[[1u73]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1u73]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bothrops_jararacussu Bothrops jararacussu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U73 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U73 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u73 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u73 OCA], [https://pdbe.org/1u73 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u73 RCSB], [https://www.ebi.ac.uk/pdbsum/1u73 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u73 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PA2A_BOTJR PA2A_BOTJR] Snake venom phospholipase A2 (PLA2) that induces edema (activity that is inhibited by EDTA and dexamethasone), inhibits phospholipid-dependent collagen/ADP-induced platelet aggregation, possess hypotensive as well as anticoagulant activities. In addition, this enzyme shows bactericidal activity against E.coli and S.aureus. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.<ref>PMID:12167491</ref> <ref>PMID:15214498</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u7/1u73_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u73 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1U73 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bothrops_jararacussu Bothrops jararacussu]. Active as [http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1U73 OCA].
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*[[Phospholipase A2 3D structures|Phospholipase A2 3D structures]]
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== References ==
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==Reference==
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<references/>
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Crystal structure of an acidic platelet aggregation inhibitor and hypotensive phospholipase A2 in the monomeric and dimeric states: insights into its oligomeric state., Magro AJ, Murakami MT, Marcussi S, Soares AM, Arni RK, Fontes MR, Biochem Biophys Res Commun. 2004 Oct 8;323(1):24-31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15351695 15351695]
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__TOC__
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</StructureSection>
[[Category: Bothrops jararacussu]]
[[Category: Bothrops jararacussu]]
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[[Category: Phospholipase A(2)]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Arni RK]]
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[[Category: Arni, R.K.]]
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[[Category: Fontes MR]]
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[[Category: Fontes, M.R.]]
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[[Category: Magro AJ]]
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[[Category: Magro, A.J.]]
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[[Category: Murakami MT]]
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[[Category: Murakami, M.T.]]
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[[Category: Soares AM]]
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[[Category: Soares, A.M.]]
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[[Category: acidic phospholipase a2]]
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[[Category: bothrops jararacussu venom]]
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[[Category: crystal structure]]
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[[Category: dimeric]]
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[[Category: oligomeric state]]
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[[Category: platelet aggregation and hypotensive effects]]
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[[Category: x-ray crystallography]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 03:26:35 2007''
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Current revision

Crystal structure of a Dimeric Acidic Platelet Aggregation Inhibitor and Hypotensive Phospholipase A2 from Bothrops jararacussu

PDB ID 1u73

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