1q3i

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(New page: 200px<br /><applet load="1q3i" size="450" color="white" frame="true" align="right" spinBox="true" caption="1q3i, resolution 2.6&Aring;" /> '''Crystal Structure of ...)
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[[Image:1q3i.jpg|left|200px]]<br /><applet load="1q3i" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1q3i, resolution 2.6&Aring;" />
 
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'''Crystal Structure of Na,K-ATPase N-domain'''<br />
 
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==Overview==
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==Crystal Structure of Na,K-ATPase N-domain==
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The structure of the N-domain of porcine alpha(2) Na,K-ATPase was, determined crystallographically to 3.2A resolution by isomorphous, heavy-atom replacement using a single mercury derivative. The structure, was finally refined against 2.6A resolution synchrotron data. The domain, forms a seven-stranded antiparallel beta-sheet with two additional, beta-strands forming a hairpin and five alpha-helices. Approximately 75%, of the residues were superimposable with residues from the structure of, Ca-ATPase N-domain, and a structure-based sequence alignment is presented., The positions of key residues are discussed in relation to the pattern of, hydrophobicity, charge and sequence conservation of the molecular surface., The structure of a hexahistidine tag binding to nickel ions is presented.
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<StructureSection load='1q3i' size='340' side='right'caption='[[1q3i]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1q3i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q3I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q3I FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q3i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q3i OCA], [https://pdbe.org/1q3i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q3i RCSB], [https://www.ebi.ac.uk/pdbsum/1q3i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q3i ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AT1A2_PIG AT1A2_PIG] This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium, providing the energy for active transport of various nutrients (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q3/1q3i_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1q3i ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1Q3I is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with NI as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Sodium/potassium-exchanging_ATPase Sodium/potassium-exchanging ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.9 3.6.3.9] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Q3I OCA].
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*[[ATPase 3D structures|ATPase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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The crystallographic structure of Na,K-ATPase N-domain at 2.6A resolution., Hakansson KO, J Mol Biol. 2003 Oct 3;332(5):1175-82. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14499619 14499619]
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[[Category: Large Structures]]
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[[Category: Protein complex]]
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[[Category: Sodium/potassium-exchanging ATPase]]
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[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
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[[Category: Hakansson, K.O.]]
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[[Category: Hakansson KO]]
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[[Category: NI]]
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[[Category: anti-parallel beta sheet]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 03:30:29 2007''
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Current revision

Crystal Structure of Na,K-ATPase N-domain

PDB ID 1q3i

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