1uai

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(New page: 200px<br /><applet load="1uai" size="450" color="white" frame="true" align="right" spinBox="true" caption="1uai, resolution 1.20&Aring;" /> '''Crystal Structure of...)
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[[Image:1uai.jpg|left|200px]]<br /><applet load="1uai" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1uai, resolution 1.20&Aring;" />
 
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'''Crystal Structure of the Alginate Lyase from Corynebacterium sp.'''<br />
 
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==Overview==
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==Crystal Structure of the Alginate Lyase from Corynebacterium sp.==
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The crystal structure of alginate (poly alpha-l-guluronate) lyase from, Corynebacterium sp. (ALY-1) was determined at 1.2A resolution using the, MAD method and bromide ions. The structure of ALY-1 is abundant in, beta-strands and has a deep cleft, similar to the jellyroll beta-sandwich, found in 1,3-1,4-beta-glucanase. The structure suggests that alginate, molecules may penetrate into the cleft to interact with the catalytic site, of ALY-1. The reported crystal structure of another type of alginate, lyase, A1-III, differs from that of ALY-1 in that it consists almost, entirely of alpha-helical structure. Nevertheless, the putative catalytic, residues in both enzymes are positioned in space in nearly identical, arrangements. This finding suggests that both alginate lyases may have, evolved through convergent evolution.
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<StructureSection load='1uai' size='340' side='right'caption='[[1uai]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1uai]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_sp. Corynebacterium sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UAI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UAI FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uai FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uai OCA], [https://pdbe.org/1uai PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uai RCSB], [https://www.ebi.ac.uk/pdbsum/1uai PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uai ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9RB42_9CORY Q9RB42_9CORY]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ua/1uai_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1uai ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of alginate (poly alpha-l-guluronate) lyase from Corynebacterium sp. (ALY-1) was determined at 1.2A resolution using the MAD method and bromide ions. The structure of ALY-1 is abundant in beta-strands and has a deep cleft, similar to the jellyroll beta-sandwich found in 1,3-1,4-beta-glucanase. The structure suggests that alginate molecules may penetrate into the cleft to interact with the catalytic site of ALY-1. The reported crystal structure of another type of alginate lyase, A1-III, differs from that of ALY-1 in that it consists almost entirely of alpha-helical structure. Nevertheless, the putative catalytic residues in both enzymes are positioned in space in nearly identical arrangements. This finding suggests that both alginate lyases may have evolved through convergent evolution.
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==About this Structure==
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Crystal structure of the alginate (poly alpha-l-guluronate) lyase from Corynebacterium sp. at 1.2 A resolution.,Osawa T, Matsubara Y, Muramatsu T, Kimura M, Kakuta Y J Mol Biol. 2005 Feb 4;345(5):1111-8. Epub 2004 Dec 13. PMID:15644208<ref>PMID:15644208</ref>
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1UAI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Corynebacterium_sp. Corynebacterium sp.]. Active as [http://en.wikipedia.org/wiki/Poly(alpha-L-guluronate)_lyase Poly(alpha-L-guluronate) lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.11 4.2.2.11] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UAI OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure of the alginate (poly alpha-l-guluronate) lyase from Corynebacterium sp. at 1.2 A resolution., Osawa T, Matsubara Y, Muramatsu T, Kimura M, Kakuta Y, J Mol Biol. 2005 Feb 4;345(5):1111-8. Epub 2004 Dec 13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15644208 15644208]
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</div>
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[[Category: Corynebacterium sp.]]
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<div class="pdbe-citations 1uai" style="background-color:#fffaf0;"></div>
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[[Category: Poly(alpha-L-guluronate) lyase]]
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== References ==
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[[Category: Single protein]]
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<references/>
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[[Category: Kakuta, Y.]]
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__TOC__
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[[Category: jellyroll beta-sandwich]]
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</StructureSection>
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[[Category: Corynebacterium sp]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 03:37:35 2007''
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[[Category: Large Structures]]
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[[Category: Kakuta Y]]

Current revision

Crystal Structure of the Alginate Lyase from Corynebacterium sp.

PDB ID 1uai

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