1duh

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(New page: 200px<br /><applet load="1duh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1duh, resolution 2.70&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1duh.gif|left|200px]]<br /><applet load="1duh" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1duh, resolution 2.70&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE CONSERVED DOMAIN IV OF E. COLI 4.5S RNA'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF THE CONSERVED DOMAIN IV OF E. COLI 4.5S RNA==
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BACKGROUND: Bacterial signal recognition particle (SRP), consisting of, 4.5S RNA and Ffh protein, plays an essential role in targeting, signal-peptide-containing proteins to the secretory apparatus in the cell, membrane. The 4.5S RNA increases the affinity of Ffh for signal peptides, and is essential for the interaction between SRP and its receptor, protein, FtsY. The 4.5S RNA also interacts with elongation factor G (EF-G) in the, ribosome and this interaction is required for efficient translation., RESULTS: We have determined by multiple anomalous dispersion (MAD) with, Lu(3+) the 2.7 A crystal structure of a 4.5S RNA fragment containing, binding sites for both Ffh and EF-G. This fragment consists of three, helices connected by a symmetric and an asymmetric internal loop. In, contrast to NMR-derived structures reported previously, the symmetric loop, is entirely constituted by non-canonical base pairs. These pairs, continuously stack and project unusual sets of hydrogen-bond donors and, acceptors into the shallow minor groove. The structure can therefore be, regarded as two double helical rods hinged by the asymmetric loop that, protrudes from one strand. CONCLUSIONS: Based on our crystal structure and, results of chemical protection experiments reported previously, we, predicted that Ffh binds to the minor groove of the symmetric loop. An, identical decanucleotide sequence is found in the EF-G binding sites of, both 4.5S RNA and 23S rRNA. The decanucleotide structure in the 4.5S RNA, and the ribosomal protein L11-RNA complex crystals suggests how 4.5S RNA, and 23S rRNA might interact with EF-G and function in translating, ribosomes.
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<StructureSection load='1duh' size='340' side='right'caption='[[1duh]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1duh]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DUH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DUH FirstGlance]. <br>
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1DUH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with LU, MG and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DUH OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LU:LUTETIUM+(III)+ION'>LU</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PGP:GUANOSINE-3,5-DIPHOSPHATE'>PGP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1duh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1duh OCA], [https://pdbe.org/1duh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1duh RCSB], [https://www.ebi.ac.uk/pdbsum/1duh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1duh ProSAT]</span></td></tr>
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Crystal structure of the ffh and EF-G binding sites in the conserved domain IV of Escherichia coli 4.5S RNA., Jovine L, Hainzl T, Oubridge C, Scott WG, Li J, Sixma TK, Wonacott A, Skarzynski T, Nagai K, Structure. 2000 May 15;8(5):527-40. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10801497 10801497]
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</table>
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[[Category: Single protein]]
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__TOC__
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[[Category: Hainzl, T.]]
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</StructureSection>
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[[Category: Jovine, L.]]
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[[Category: Large Structures]]
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[[Category: Li, J.]]
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[[Category: Hainzl T]]
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[[Category: Nagai, K.]]
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[[Category: Jovine L]]
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[[Category: Oubridge, C.]]
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[[Category: Li J]]
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[[Category: Scott, W.G.]]
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[[Category: Nagai K]]
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[[Category: Sixma, T.K.]]
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[[Category: Oubridge C]]
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[[Category: Skarzynski, T.]]
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[[Category: Scott WG]]
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[[Category: Wonacott, A.]]
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[[Category: Sixma TK]]
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[[Category: LU]]
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[[Category: Skarzynski T]]
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[[Category: MG]]
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[[Category: Wonacott A]]
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[[Category: SO4]]
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[[Category: 23s rna]]
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[[Category: 4.5s rna]]
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[[Category: domain iv]]
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[[Category: ef-g]]
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[[Category: elongation factor g]]
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[[Category: ffh]]
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[[Category: helix 8]]
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[[Category: mismatch]]
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[[Category: non-canonical base pairs]]
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[[Category: signal recognition particle]]
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[[Category: srp]]
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[[Category: srp54]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 03:41:47 2007''
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Current revision

CRYSTAL STRUCTURE OF THE CONSERVED DOMAIN IV OF E. COLI 4.5S RNA

PDB ID 1duh

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