3kki

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{{Seed}}
 
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[[Image:3kki.png|left|200px]]
 
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==PLP-Dependent Acyl-CoA transferase CqsA==
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The line below this paragraph, containing "STRUCTURE_3kki", creates the "Structure Box" on the page.
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<StructureSection load='3kki' size='340' side='right'caption='[[3kki]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3kki]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3hqs 3hqs]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KKI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KKI FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_3kki| PDB=3kki | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3kki FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kki OCA], [https://pdbe.org/3kki PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3kki RCSB], [https://www.ebi.ac.uk/pdbsum/3kki PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3kki ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CQSA_VIBCH CQSA_VIBCH] Required for the synthesis of the quorum-sensing autoinducer CAI-1 ((S)-3-hydroxytridecan-4-one) which probably functions as an intragenus signal.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kk/3kki_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3kki ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Vibrio cholerae, the bacterium that causes the disease cholera, controls virulence factor production and biofilm development in response to two extracellular quorum-sensing molecules, called autoinducers. The strongest autoinducer, called CAI-1 (for cholera autoinducer-1), was previously identified as (S)-3-hydroxytridecan-4-one. Biosynthesis of CAI-1 requires the enzyme CqsA. Here, we determine the CqsA reaction mechanism, identify the CqsA substrates as (S)-2-aminobutyrate and decanoyl coenzyme A, and demonstrate that the product of the reaction is 3-aminotridecan-4-one, dubbed amino-CAI-1. CqsA produces amino-CAI-1 by a pyridoxal phosphate-dependent acyl-CoA transferase reaction. Amino-CAI-1 is converted to CAI-1 in a subsequent step via a CqsA-independent mechanism. Consistent with this, we find cells release &gt; or =100 times more CAI-1 than amino-CAI-1. Nonetheless, V. cholerae responds to amino-CAI-1 as well as CAI-1, whereas other CAI-1 variants do not elicit a quorum-sensing response. Thus, both CAI-1 and amino-CAI-1 have potential as lead molecules in the development of an anticholera treatment.
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===PLP-Dependent Acyl-CoA transferase CqsA===
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The Vibrio cholerae quorum-sensing autoinducer CAI-1: analysis of the biosynthetic enzyme CqsA.,Kelly RC, Bolitho ME, Higgins DA, Lu W, Ng WL, Jeffrey PD, Rabinowitz JD, Semmelhack MF, Hughson FM, Bassler BL Nat Chem Biol. 2009 Dec;5(12):891-5. Epub 2009 Oct 18. PMID:19838203<ref>PMID:19838203</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19838203}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3kki" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19838203 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19838203}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3KKI is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3hqs 3hqs]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KKI OCA].
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==Reference==
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<ref group="xtra">PMID:19838203</ref><references group="xtra"/>
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[[Category: Vibrio cholerae]]
[[Category: Vibrio cholerae]]
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[[Category: Hughson, F M.]]
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[[Category: Hughson FM]]
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[[Category: Jeffrey, P D.]]
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[[Category: Jeffrey PD]]
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[[Category: Kelly, R C.]]
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[[Category: Kelly RC]]
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[[Category: Acyltransferase]]
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[[Category: Aminotransferase]]
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[[Category: Autoinducer]]
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[[Category: Cai-1]]
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[[Category: Cqsa]]
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[[Category: Plp]]
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[[Category: Pyridoxal phosphate]]
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[[Category: Quorum sensing]]
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[[Category: Transferase]]
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[[Category: Vibrio cholerae]]
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[[Category: Virulence]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 2 09:51:18 2009''
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Current revision

PLP-Dependent Acyl-CoA transferase CqsA

PDB ID 3kki

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