3ht5

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{{Seed}}
 
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[[Image:3ht5.jpg|left|200px]]
 
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==Crystal Structure of IlvE a Branched Chain Amino Acid Transaminase from Mycobacterium tuberculosis==
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The line below this paragraph, containing "STRUCTURE_3ht5", creates the "Structure Box" on the page.
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<StructureSection load='3ht5' size='340' side='right'caption='[[3ht5]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3ht5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HT5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HT5 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PMP:4-DEOXY-4-AMINOPYRIDOXAL-5-PHOSPHATE'>PMP</scene></td></tr>
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{{STRUCTURE_3ht5| PDB=3ht5 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ht5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ht5 OCA], [https://pdbe.org/3ht5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ht5 RCSB], [https://www.ebi.ac.uk/pdbsum/3ht5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ht5 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ILVE_MYCTU ILVE_MYCTU] Catalyzes the reversible transfers of an amino group from glutamate to the alpha-ketoacid of the respective amino acid in the final step in the biosynthesis of branchedchain amino acids. The amino acids can be ranked in the following order with respect to their efficiency as amino donor: Leu > Ile > Val.<ref>PMID:20445230</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ht/3ht5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ht5 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Unlike mammals, bacteria encode enzymes that synthesize branched-chain amino acids. The pyridoxal 50-phosphate-dependent transaminase performs the final biosynthetic step in these pathways, converting keto acid precursors into -amino acids. The branched-chain amino-acid transaminase from Mycobacterium tuberculosis (MtIlvE) has been crystallized and its structure has been solved at 1.9 angstrom resolution. The MtIlvE monomer is composed of two domains that interact to form the active site. The biologically active form of IlvE is a homodimer in which each monomer contributes a substrate-specificity loop to the partner molecule. Additional substrate selectivity may be imparted by a conserved N-terminal Phe30 residue, which has previously been observed to shield the active site in the type IV fold homodimer. The active site of MtIlvE contains density corresponding to bound PMP, which is likely to be a consequence of the presence of tryptone in the crystallization medium. Additionally, two cysteine residues are positioned at the dimer interface for disulfide-bond formation under oxidative conditions. It is unknown whether they are involved in any regulatory activities analogous to those of the human mitochondrial branched-chain amino-acid transaminase.
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===Crystal Structure of IlvE a Branched Chain Amino Acid Transaminase from Mycobacterium tuberculosis===
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The 1.9 A structure of the branched-chain amino-acid transaminase (IlvE) from Mycobacterium tuberculosis.,Tremblay LW, Blanchard JS Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Nov 1;65(Pt, 11):1071-7. Epub 2009 Oct 13. PMID:19923721<ref>PMID:19923721</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19923721}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3ht5" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19923721 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19923721}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3HT5 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HT5 OCA].
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==Reference==
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<ref group="xtra">PMID:19923721</ref><references group="xtra"/>
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[[Category: Branched-chain-amino-acid transaminase]]
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[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
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[[Category: Blanchard, J S.]]
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[[Category: Blanchard JS]]
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[[Category: Tremblay, L W.]]
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[[Category: Tremblay LW]]
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[[Category: Amino-acid biosynthesis]]
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[[Category: Aminotransferase]]
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[[Category: Branched-chain amino acid biosynthesis]]
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[[Category: Branched-chain-amino-acid]]
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[[Category: Plp binding type iv fold]]
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[[Category: Pyridoxal phosphate]]
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[[Category: Tb]]
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[[Category: Transaminase]]
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[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 2 10:01:36 2009''
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Current revision

Crystal Structure of IlvE a Branched Chain Amino Acid Transaminase from Mycobacterium tuberculosis

PDB ID 3ht5

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