1dxj
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(New page: 200px<br /><applet load="1dxj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dxj, resolution 1.8Å" /> '''STRUCTURE OF THE CHIT...) |
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| - | [[Image:1dxj.gif|left|200px]]<br /><applet load="1dxj" size="450" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="1dxj, resolution 1.8Å" /> | ||
| - | '''STRUCTURE OF THE CHITINASE FROM JACK BEAN'''<br /> | ||
| - | == | + | ==Structure of the chitinase from jack bean== |
| - | The structure of jack bean chitinase was solved at 1.8 A resolution by | + | <StructureSection load='1dxj' size='340' side='right'caption='[[1dxj]], [[Resolution|resolution]] 1.80Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1dxj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Canavalia_ensiformis Canavalia ensiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DXJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DXJ FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dxj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dxj OCA], [https://pdbe.org/1dxj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dxj RCSB], [https://www.ebi.ac.uk/pdbsum/1dxj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dxj ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/O81934_CANEN O81934_CANEN] | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dx/1dxj_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dxj ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The structure of jack bean chitinase was solved at 1.8 A resolution by molecular replacement. It is an alpha-helical protein with three disulfide bridges. The active site is related in structure to animal and viral lysozymes. However, unlike in lysozyme, the architecture of the active site suggests a single-step cleavage. According to this mechanism, Glu68 is the proton donor and Glu90 assists in the reaction by moving towards the substrate and recruiting a water molecule that acts as the nucleophile. In this model, a water molecule was found in contact with Glu90 O(epsilon1) and Thr119 O(gamma) at a distance of 3.0 and 2.8 A, respectively. The model is in accordance with the observed inversion mechanism. | ||
| - | + | Structure of jack bean chitinase.,Hahn M, Hennig M, Schlesier B, Hohne W Acta Crystallogr D Biol Crystallogr. 2000 Sep;56(Pt 9):1096-9. PMID:10957628<ref>PMID:10957628</ref> | |
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| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 1dxj" style="background-color:#fffaf0;"></div> | |
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| - | + | ==See Also== | |
| + | *[[Chitinase 3D structures|Chitinase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Canavalia ensiformis]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Hahn M]] | ||
| + | [[Category: Hennig M]] | ||
| + | [[Category: Hohne W]] | ||
| + | [[Category: Schlesier B]] | ||
Current revision
Structure of the chitinase from jack bean
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