1i9g

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(New page: 200px<br /><applet load="1i9g" size="450" color="white" frame="true" align="right" spinBox="true" caption="1i9g, resolution 1.98&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1i9g.gif|left|200px]]<br /><applet load="1i9g" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1i9g, resolution 1.98&Aring;" />
 
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'''CRYSTAL STRUCTURE OF AN ADOMET DEPENDENT METHYLTRANSFERASE'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF AN ADOMET DEPENDENT METHYLTRANSFERASE==
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Rv2118c belongs to the class of conserved hypothetical proteins from, Mycobacterium tuberculosis H37Rv. The crystal structure of Rv2118c in, complex with S-adenosyl-l-methionine (AdoMet) has been determined at 1.98, A resolution. The crystallographic asymmetric unit consists of a monomer, but symmetry-related subunits interact extensively, leading to a, tetrameric structure. The structure of the monomer can be divided, functionally into two domains: the larger catalytic C-terminal domain that, binds the cofactor AdoMet and is involved in the transfer of methyl group, from AdoMet to the substrate and a smaller N-terminal domain. The, structure of the catalytic domain is very similar to that of other, AdoMet-dependent methyltransferases. The N-terminal domain is primarily a, beta-structure with a fold not found in other methyltransferases of known, structure. Database searches reveal a conserved family of Rv2118c-like, proteins from various organisms. Multiple sequence alignments show several, regions of high sequence similarity (motifs) in this family of proteins., Structure analysis and homology to yeast Gcd14p suggest that Rv2118c could, be an RNA methyltransferase, but further studies are required to establish, its functional role conclusively. Copyright 12001 Academic Press.
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<StructureSection load='1i9g' size='340' side='right'caption='[[1i9g]], [[Resolution|resolution]] 1.98&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1i9g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I9G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I9G FirstGlance]. <br>
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1I9G is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with SAM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1I9G OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.98&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i9g OCA], [https://pdbe.org/1i9g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i9g RCSB], [https://www.ebi.ac.uk/pdbsum/1i9g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i9g ProSAT]</span></td></tr>
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Crystal structure of Rv2118c: an AdoMet-dependent methyltransferase from Mycobacterium tuberculosis H37Rv., Gupta A, Kumar PH, Dineshkumar TK, Varshney U, Subramanya HS, J Mol Biol. 2001 Sep 14;312(2):381-91. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11554794 11554794]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TRMI_MYCTU TRMI_MYCTU] Catalyzes the S-adenosyl-L-methionine-dependent formation of N(1)-methyladenine at position 58 (m1A58) in tRNA.<ref>PMID:14960715</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i9/1i9g_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1i9g ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
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[[Category: Single protein]]
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[[Category: Dineshkumar TK]]
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[[Category: Dineshkumar, T.K.]]
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[[Category: Gupta A]]
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[[Category: Gupta, A.]]
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[[Category: Kumar PH]]
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[[Category: Kumar, P.H.]]
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[[Category: Subramanya HS]]
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[[Category: Subramanya, H.S.]]
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[[Category: Varshney U]]
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[[Category: TBSGC, TB.Structural.Genomics.Consortium.]]
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[[Category: Varshney, U.]]
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[[Category: SAM]]
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[[Category: adomet]]
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[[Category: crystal]]
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[[Category: mtase]]
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[[Category: mycobacterium]]
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[[Category: protein structure initiative]]
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[[Category: psi]]
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[[Category: structural genomics]]
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[[Category: tb structural genomics consortium]]
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[[Category: tbsgc]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 03:48:34 2007''
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Current revision

CRYSTAL STRUCTURE OF AN ADOMET DEPENDENT METHYLTRANSFERASE

PDB ID 1i9g

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