3ite

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{{Seed}}
 
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[[Image:3ite.png|left|200px]]
 
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==The third adenylation domain of the fungal SidN non-ribosomal peptide synthetase==
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The line below this paragraph, containing "STRUCTURE_3ite", creates the "Structure Box" on the page.
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<StructureSection load='3ite' size='340' side='right'caption='[[3ite]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3ite]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Epichloe_festucae_var._lolii Epichloe festucae var. lolii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ITE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ITE FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_3ite| PDB=3ite | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ite FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ite OCA], [https://pdbe.org/3ite PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ite RCSB], [https://www.ebi.ac.uk/pdbsum/3ite PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ite ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SIDN_EPIFI SIDN_EPIFI] Nonribosomal peptide synthase required for the biosynthetis of epichloenin A, an extracellular siderophore that plays a crucial role in endophyte-grass symbioses (PubMed:19923209, PubMed:23658520). SidN assembles epichloenin A by activating and incorporating three trans-anhydromevalonylhydroxyornithine (trans-AMHO), 1 glutamine and 4 glycine moieties (PubMed:23658520). Trans-AMHO is produced from L-ornithine via 2 steps involving a L-ornithine N(5)-monooxygenase and an AHMO-N(5)-transacylase that have still to be identified (PubMed:19923209). The third adenylation domain (A3) of sidN incorporates the hydroxamate groups of the siderophore which forms an octahedral iron complex (PubMed:19923209). The other component amino acids are assembled by sidN adenylation domains A1 and A2 (PubMed:19923209, PubMed:23658520).<ref>PMID:19923209</ref> <ref>PMID:23658520</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/it/3ite_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ite ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Nonribosomal peptide synthetases (NRPSs) are large, multidomain proteins that are involved in the biosynthesis of an array of secondary metabolites. We report the structure of the third adenylation domain from the siderophore-synthesizing NRPS, SidN, from the endophytic fungus Neotyphodium lolii. This is the first structure of a eukaryotic NRPS domain, and it reveals a large binding pocket required to accommodate the unusual amino acid substrate, N(delta)-cis-anhydromevalonyl-N(delta)-hydroxy-L-ornithine (cis-AMHO). The specific activation of cis-AMHO was confirmed biochemically, and an AMHO moiety was unambiguously identified as a component of the fungal siderophore using mass spectroscopy. The protein structure shows that the substrate binding pocket is defined by 17 amino acid residues, in contrast to both prokaryotic adenylation domains and to previous predictions based on modeling. Existing substrate prediction methods for NRPS adenylation domains fail for domains from eukaryotes due to the divergence of their signature sequences from those of prokaryotes. Thus, this new structure will provide a basis for improving prediction methods for eukaryotic NRPS enzymes that play important and diverse roles in the biology of fungi.
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===The third adenylation domain of the fungal SidN non-ribosomal peptide synthetase===
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Structure of a eukaryotic nonribosomal peptide synthetase adenylation domain that activates a large hydroxamate amino acid in siderophore biosynthesis.,Lee TV, Johnson LJ, Johnson RD, Koulman A, Lane GA, Lott JS, Arcus VL J Biol Chem. 2010 Jan 22;285(4):2415-27. Epub 2009 Nov 18. PMID:19923209<ref>PMID:19923209</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The line below this paragraph, {{ABSTRACT_PUBMED_19923209}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3ite" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19923209 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19923209}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Epichloe festucae var. lolii]]
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3ITE is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Neotyphodium_lolii Neotyphodium lolii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ITE OCA].
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[[Category: Large Structures]]
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[[Category: Arcus VL]]
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==Reference==
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[[Category: Johnson LJ]]
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<ref group="xtra">PMID:19923209</ref><references group="xtra"/>
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[[Category: Johnson RD]]
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[[Category: Neotyphodium lolii]]
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[[Category: Lee TV]]
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[[Category: Arcus, V L.]]
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[[Category: Lott JS]]
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[[Category: Johnson, L J.]]
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[[Category: Johnson, R D.]]
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[[Category: Lee, T V.]]
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[[Category: Lott, J S.]]
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[[Category: Endophyte]]
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[[Category: Fungal]]
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[[Category: Ligase]]
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[[Category: Non-ribosomal peptide synthesis]]
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[[Category: Nrp]]
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[[Category: Siderophore synthetase]]
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[[Category: Sidna3]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 9 14:23:26 2009''
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Current revision

The third adenylation domain of the fungal SidN non-ribosomal peptide synthetase

PDB ID 3ite

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