1ybk

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(New page: 200px<br /><applet load="1ybk" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ybk, resolution 1.45&Aring;" /> '''RHCC cocrystallized ...)
Current revision (08:56, 14 February 2024) (edit) (undo)
 
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[[Image:1ybk.gif|left|200px]]<br /><applet load="1ybk" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ybk, resolution 1.45&Aring;" />
 
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'''RHCC cocrystallized with CAPB'''<br />
 
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==Overview==
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==RHCC cocrystallized with CAPB==
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Crystals of excellent quality are a prerequisite for high-resolution X-ray, data. However, in refinement protocols of crystallization conditions it is, often difficult to obtain the right combination of, for example, protein, concentration, drop size, temperature and additives. A novel approach for, optimizing crystal contacts in a most favourable fashion by performing, crystallization setups with the zwitterionic surfactant, cocoamidoproylbetaine (CAPB) is introduced. In the presence of this, surfactant, highly diffracting crystals were obtained. Here, data from a, right-handed coiled coil (RHCC) in complex with CAPB at 1.4 A resolution, are presented. The addition of CAPB using otherwise identical, crystallization conditions and the same X-ray source caused an improvement, in resolution from 2.9 to 1.4 A.
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<StructureSection load='1ybk' size='340' side='right'caption='[[1ybk]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1ybk]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylothermus_marinus Staphylothermus marinus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YBK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YBK FirstGlance]. <br>
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1YBK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylothermus_marinus Staphylothermus marinus] with SO4 and BEQ as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1YBK OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEQ:N-(CARBOXYMETHYL)-N,N-DIMETHYL-3-[(1-OXODODECYL)AMINO]-1-PROPANAMINIUM+INNER+SALT'>BEQ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ybk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ybk OCA], [https://pdbe.org/1ybk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ybk RCSB], [https://www.ebi.ac.uk/pdbsum/1ybk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ybk ProSAT]</span></td></tr>
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Favourable mediation of crystal contacts by cocoamidopropylbetaine (CAPB)., Ozbek S, Muller JF, Figgemeier E, Stetefeld J, Acta Crystallogr D Biol Crystallogr. 2005 Apr;61(Pt 4):477-80. Epub 2005, Mar 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15805603 15805603]
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</table>
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[[Category: Single protein]]
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== Function ==
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[https://www.uniprot.org/uniprot/Q54436_STAMA Q54436_STAMA]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Staphylothermus marinus]]
[[Category: Staphylothermus marinus]]
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[[Category: Stetefeld, J.]]
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[[Category: Stetefeld J]]
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[[Category: BEQ]]
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[[Category: SO4]]
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[[Category: right hand coiled coil]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 03:54:31 2007''
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Current revision

RHCC cocrystallized with CAPB

PDB ID 1ybk

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