2v57

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{{Seed}}
 
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[[Image:2v57.png|left|200px]]
 
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==Crystal structure of the TetR-like transcriptional regulator LfrR from Mycobacterium smegmatis in complex with proflavine==
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The line below this paragraph, containing "STRUCTURE_2v57", creates the "Structure Box" on the page.
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<StructureSection load='2v57' size='340' side='right'caption='[[2v57]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2v57]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis Mycolicibacterium smegmatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V57 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V57 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=PRL:PROFLAVIN'>PRL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_2v57| PDB=2v57 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v57 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v57 OCA], [https://pdbe.org/2v57 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v57 RCSB], [https://www.ebi.ac.uk/pdbsum/2v57 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v57 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q58L87_MYCSM Q58L87_MYCSM]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v5/2v57_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2v57 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The TetR-like transcriptional repressor LfrR controls the expression of the gene encoding the Mycobacterium smegmatis efflux pump LfrA, which actively extrudes fluoroquinolones, cationic dyes, and anthracyclines from the cell and promotes intrinsic antibiotic resistance. The crystal structure of the apoprotein form of the repressor reveals a structurally asymmetric homodimer exhibiting local unfolding and a blocked drug-binding site, emphasizing the significant conformational plasticity of the protein necessary for DNA and multidrug recognition. Crystallographic and calorimetric studies of LfrR-drug complexes further confirm the intrinsic flexibility of the homodimer, which provides a dynamic mechanism to broaden multidrug binding specificity and may be a general property of transcriptional repressors regulating microbial efflux pump expression.
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===CRYSTAL STRUCTURE OF THE TETR-LIKE TRANSCRIPTIONAL REGULATOR LFRR FROM MYCOBACTERIUM SMEGMATIS IN COMPLEX WITH PROFLAVINE===
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Structural plasticity and distinct drug-binding modes of LfrR, a mycobacterial efflux pump regulator.,Bellinzoni M, Buroni S, Schaeffer F, Riccardi G, De Rossi E, Alzari PM J Bacteriol. 2009 Dec;191(24):7531-7. Epub 2009 Oct 9. PMID:19820093<ref>PMID:19820093</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19820093}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2v57" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19820093 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19820093}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2V57 is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis Mycobacterium smegmatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V57 OCA].
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[[Category: Mycolicibacterium smegmatis]]
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[[Category: Alzari PM]]
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==Reference==
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[[Category: Bellinzoni M]]
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<ref group="xtra">PMID:19820093</ref><references group="xtra"/>
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[[Category: Buroni S]]
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[[Category: Mycobacterium smegmatis]]
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[[Category: De Rossi E]]
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[[Category: Alzari, P M.]]
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[[Category: Riccardi G]]
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[[Category: Bellinzoni, M.]]
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[[Category: Buroni, S.]]
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[[Category: Riccardi, G.]]
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[[Category: Rossi, E De.]]
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[[Category: Dna-binding]]
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[[Category: Lfrr]]
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[[Category: Repressor]]
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[[Category: Tetr]]
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[[Category: Transcription]]
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[[Category: Transcription regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 9 14:34:30 2009''
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Current revision

Crystal structure of the TetR-like transcriptional regulator LfrR from Mycobacterium smegmatis in complex with proflavine

PDB ID 2v57

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