1m8n

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(New page: 200px<br /><applet load="1m8n" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m8n, resolution 2.45&Aring;" /> '''Choristoneura Fumife...)
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[[Image:1m8n.gif|left|200px]]<br /><applet load="1m8n" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1m8n, resolution 2.45&Aring;" />
 
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'''Choristoneura Fumiferana (Spruce Budworm) Antifreeze Protein Isoform 501'''<br />
 
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==Overview==
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==Choristoneura Fumiferana (Spruce Budworm) Antifreeze Protein Isoform 501==
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The insect spruce budworm (Choristoneura fumiferana)(Cf) produces a number, of isoforms of its highly active antifreeze protein (CfAFP). Although most, of the CfAFP isoforms are in the 9-kDa range, isoforms containing a 30- or, 31-amino acid insertion have also been identified. Here we describe the, functional and structural analysis of a selected long isoform, CfAFP-501., X-ray crystal structure determination reveals that the 31-amino acid, insertion found in CfAFP-501 forms two additional loops within its highly, regular beta-helical structure. This effectively extends the area of the, two-dimensional Thr array and ice-binding surface of the protein. The, larger isoform has 3 times the thermal hysteresis activity of the 9-kDa, CfAFP-337. As well, a deletion of the 31-amino acid insertion within, CfAFP-501 to form CfAFP-501-Delta-2-loop, results in a protein with, reduced activity similar to the shorter CfAFP isoforms. Thus, the enhanced, antifreeze activity of CfAFP-501 is directly correlated to the length of, its beta-helical structure and hence the size of its ice-binding face.
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<StructureSection load='1m8n' size='340' side='right'caption='[[1m8n]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1m8n]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Choristoneura_fumiferana Choristoneura fumiferana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M8N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M8N FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m8n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m8n OCA], [https://pdbe.org/1m8n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m8n RCSB], [https://www.ebi.ac.uk/pdbsum/1m8n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m8n ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9GSA6_CHOFU Q9GSA6_CHOFU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m8/1m8n_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m8n ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1M8N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Choristoneura_fumiferana Choristoneura fumiferana]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1M8N OCA].
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*[[Antifreeze protein 3D structures|Antifreeze protein 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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A beta-helical antifreeze protein isoform with increased activity. Structural and functional insights., Leinala EK, Davies PL, Doucet D, Tyshenko MG, Walker VK, Jia Z, J Biol Chem. 2002 Sep 6;277(36):33349-52. Epub 2002 Jun 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12105229 12105229]
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[[Category: Choristoneura fumiferana]]
[[Category: Choristoneura fumiferana]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Davies, P.L.]]
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[[Category: Davies PL]]
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[[Category: Jia, Z.]]
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[[Category: Jia Z]]
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[[Category: Leinala, E.K.]]
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[[Category: Leinala EK]]
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[[Category: antifreeze protein]]
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[[Category: left-handed beta-helix]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 03:56:41 2007''
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Current revision

Choristoneura Fumiferana (Spruce Budworm) Antifreeze Protein Isoform 501

PDB ID 1m8n

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