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1ugl

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(New page: 200px<br /><applet load="1ugl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ugl" /> '''Solution structure of S8-SP11'''<br /> ==Ov...)
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[[Image:1ugl.jpg|left|200px]]<br /><applet load="1ugl" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ugl" />
 
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'''Solution structure of S8-SP11'''<br />
 
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==Overview==
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==Solution structure of S8-SP11==
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Many flowering plants possess a self-incompatibility system to prevent, inbreeding. In Brassica rapa, self/non-self recognition in mating is, established through S-haplotype-specific interactions between stigma, receptors and S-locus protein 11 (SP11, also called S-locus cysteine-rich, protein) that is encoded at the highly polymorphic S-locus. Here we, describe the solution structure of the SP11 protein of the S8-haplotype, (S8-SP11), which specifically binds to the stigma factor of the same, haplotype. It folds into an alpha/beta sandwich structure that resembles, those of plant defensins. Residues important for structural integrity are, highly conserved among the allelic SP11s, suggesting the existence of a, common folding pattern. Structure-based sequence alignment and homology, modeling of allelic SP11 identified a hyper-variable (HV) region, which is, thought to form a loop that bulges out from the body of the protein that, is amenable to solvent exposure. We suggest that the HV region could serve, as a specific binding site for the stigma receptor.
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<StructureSection load='1ugl' size='340' side='right'caption='[[1ugl]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ugl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Brassica_rapa Brassica rapa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UGL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UGL FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ugl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ugl OCA], [https://pdbe.org/1ugl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ugl RCSB], [https://www.ebi.ac.uk/pdbsum/1ugl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ugl ProSAT], [https://www.topsan.org/Proteins/RSGI/1ugl TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9SE17_BRACM Q9SE17_BRACM]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Many flowering plants possess a self-incompatibility system to prevent inbreeding. In Brassica rapa, self/non-self recognition in mating is established through S-haplotype-specific interactions between stigma receptors and S-locus protein 11 (SP11, also called S-locus cysteine-rich protein) that is encoded at the highly polymorphic S-locus. Here we describe the solution structure of the SP11 protein of the S8-haplotype (S8-SP11), which specifically binds to the stigma factor of the same haplotype. It folds into an alpha/beta sandwich structure that resembles those of plant defensins. Residues important for structural integrity are highly conserved among the allelic SP11s, suggesting the existence of a common folding pattern. Structure-based sequence alignment and homology modeling of allelic SP11 identified a hyper-variable (HV) region, which is thought to form a loop that bulges out from the body of the protein that is amenable to solvent exposure. We suggest that the HV region could serve as a specific binding site for the stigma receptor.
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==About this Structure==
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Structure of the male determinant factor for Brassica self-incompatibility.,Mishima M, Takayama S, Sasaki K, Jee JG, Kojima C, Isogai A, Shirakawa M J Biol Chem. 2003 Sep 19;278(38):36389-95. Epub 2003 Jun 30. PMID:12835321<ref>PMID:12835321</ref>
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1UGL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UGL OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure of the male determinant factor for Brassica self-incompatibility., Mishima M, Takayama S, Sasaki K, Jee JG, Kojima C, Isogai A, Shirakawa M, J Biol Chem. 2003 Sep 19;278(38):36389-95. Epub 2003 Jun 30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12835321 12835321]
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</div>
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[[Category: Single protein]]
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<div class="pdbe-citations 1ugl" style="background-color:#fffaf0;"></div>
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[[Category: Isogai, A.]]
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== References ==
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[[Category: Jee, J.G.]]
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<references/>
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[[Category: Kojima, C.]]
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__TOC__
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[[Category: Mishima, M.]]
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</StructureSection>
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[[Category: RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.]]
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[[Category: Brassica rapa]]
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[[Category: Sasaki, K.]]
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[[Category: Large Structures]]
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[[Category: Shirakawa, M.]]
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[[Category: Isogai A]]
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[[Category: Takayama, S.]]
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[[Category: Jee JG]]
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[[Category: cysteine-rich]]
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[[Category: Kojima C]]
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[[Category: defensin-like]]
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[[Category: Mishima M]]
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[[Category: male determinant of self-incompatibility]]
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[[Category: Sasaki K]]
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[[Category: nmr]]
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[[Category: Shirakawa M]]
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[[Category: riken structural genomics/proteomics initiative]]
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[[Category: Takayama S]]
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[[Category: rsgi]]
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[[Category: scr]]
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[[Category: sp11]]
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[[Category: structural genomics]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 03:58:01 2007''
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Current revision

Solution structure of S8-SP11

PDB ID 1ugl

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