3k1k

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{{Seed}}
 
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[[Image:3k1k.jpg|left|200px]]
 
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==Green fluorescent protein bound to enhancer nanobody==
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The line below this paragraph, containing "STRUCTURE_3k1k", creates the "Structure Box" on the page.
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<StructureSection load='3k1k' size='340' side='right'caption='[[3k1k]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3k1k]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria] and [https://en.wikipedia.org/wiki/Camelus_dromedarius Camelus dromedarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K1K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3K1K FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GYS:[(4Z)-2-(1-AMINO-2-HYDROXYETHYL)-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>GYS</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_3k1k| PDB=3k1k | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3k1k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k1k OCA], [https://pdbe.org/3k1k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3k1k RCSB], [https://www.ebi.ac.uk/pdbsum/3k1k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3k1k ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k1/3k1k_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3k1k ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Protein conformation is critically linked to function and often controlled by interactions with regulatory factors. Here we report the selection of camelid-derived single-domain antibodies (nanobodies) that modulate the conformation and spectral properties of the green fluorescent protein (GFP). One nanobody could reversibly reduce GFP fluorescence by a factor of 5, whereas its displacement by a second nanobody caused an increase by a factor of 10. Structural analysis of GFP-nanobody complexes revealed that the two nanobodies induce subtle opposing changes in the chromophore environment, leading to altered absorption properties. Unlike conventional antibodies, the small, stable nanobodies are functional in living cells. Nanobody-induced changes were detected by ratio imaging and used to monitor protein expression and subcellular localization as well as translocation events such as the tamoxifen-induced nuclear localization of estrogen receptor. This work demonstrates that protein conformations can be manipulated and studied with nanobodies in living cells.
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===Green fluorescent protein bound to minimizer nanobody===
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Modulation of protein properties in living cells using nanobodies.,Kirchhofer A, Helma J, Schmidthals K, Frauer C, Cui S, Karcher A, Pellis M, Muyldermans S, Casas-Delucchi CS, Cardoso MC, Leonhardt H, Hopfner KP, Rothbauer U Nat Struct Mol Biol. 2010 Jan;17(1):133-8. Epub 2009 Dec 13. PMID:20010839<ref>PMID:20010839</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3k1k" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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3K1K is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria] and [http://en.wikipedia.org/wiki/Lama_pacos Lama pacos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K1K OCA].
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*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Aequorea victoria]]
[[Category: Aequorea victoria]]
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[[Category: Lama pacos]]
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[[Category: Camelus dromedarius]]
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[[Category: Cardoso, M C.]]
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[[Category: Large Structures]]
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[[Category: Cui, S.]]
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[[Category: Cardoso MC]]
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[[Category: Delucci, C C.]]
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[[Category: Cui S]]
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[[Category: Frauer, C.]]
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[[Category: Delucci CC]]
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[[Category: Helma, J.]]
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[[Category: Frauer C]]
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[[Category: Hopfner, K P.]]
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[[Category: Helma J]]
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[[Category: Karcher, A.]]
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[[Category: Hopfner K-P]]
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[[Category: Kirchhofer, A.]]
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[[Category: Karcher A]]
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[[Category: Leonhardt, H.]]
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[[Category: Kirchhofer A]]
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[[Category: Muyldermans, S.]]
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[[Category: Leonhardt H]]
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[[Category: Pellis, M.]]
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[[Category: Muyldermans S]]
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[[Category: Rothbauer, U.]]
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[[Category: Pellis M]]
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[[Category: Schmidthals, K.]]
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[[Category: Rothbauer U]]
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[[Category: Antibody-complex]]
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[[Category: Schmidthals K]]
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[[Category: Chromophore]]
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[[Category: Luminescence]]
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[[Category: Luminescent protein/immune system complex]]
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[[Category: Nanobody]]
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[[Category: Photoprotein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 9 16:04:12 2009''
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Current revision

Green fluorescent protein bound to enhancer nanobody

PDB ID 3k1k

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