2wtl

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{{Seed}}
 
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[[Image:2wtl.jpg|left|200px]]
 
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==Crystal structure of BfrA from M. tuberculosis==
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The line below this paragraph, containing "STRUCTURE_2wtl", creates the "Structure Box" on the page.
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<StructureSection load='2wtl' size='340' side='right'caption='[[2wtl]], [[Resolution|resolution]] 2.59&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2wtl]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WTL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WTL FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.59&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_2wtl| PDB=2wtl | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wtl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wtl OCA], [https://pdbe.org/2wtl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wtl RCSB], [https://www.ebi.ac.uk/pdbsum/2wtl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wtl ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BFR_MYCTU BFR_MYCTU] Iron-storage protein, whose ferroxidase center binds Fe(2+) ions, oxidizes them by dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wt/2wtl_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wtl ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Emergence of tuberculosis as a global health threat has necessitated an urgent search for new antitubercular drugs entailing determination of 3-dimensional structures of a large number of mycobacterial proteins for structure-based drug design. The essential requirement of ferritins/bacterioferritins (proteins involved in iron storage and homeostasis) for the survival of several prokaryotic pathogens makes these proteins very attractive targets for structure determination and inhibitor design. Bacterioferritins (Bfrs) differ from ferritins in that they have additional noncovalently bound haem groups. The physiological role of haem in Bfrs is not very clear but studies indicate that the haem group is involved in mediating release of iron from Bfr by facilitating reduction of the iron core. To further enhance our understanding, we have determined the crystal structure of the selenomethionyl analog of bacterioferritin A (SeMet-BfrA) from Mycobacterium tuberculosis (Mtb). Unexpectedly, electron density observed in the crystals of SeMet-BfrA analogous to haem location in bacterioferritins, shows a demetallated and degraded product of haem. This unanticipated observation is a consequence of the altered spatial electronic environment around the axial ligands of haem (in lieu of Met52 modification to SeMet52). Furthermore, the structure of Mtb SeMet-BfrA displays a possible lost protein interaction with haem propionates due to formation of a salt bridge between Arg53-Glu57, which appears to be unique to Mtb BfrA, resulting in slight modulation of haem binding pocket in this organism. The crystal structure of Mtb SeMet-BfrA provides novel leads to physiological function of haem in Bfrs. If validated as a drug target, it may also serve as a scaffold for designing specific inhibitors. In addition, this study provides evidence against the general belief that a selenium derivative of a protein represents its true physiological native structure.
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===CRYSTAL STRUCTURE OF BFRA FROM M. TUBERCULOSIS===
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Crystal structure of Bfr A from Mycobacterium tuberculosis: incorporation of selenomethionine results in cleavage and demetallation of haem.,Gupta V, Gupta RK, Khare G, Salunke DM, Tyagi AK PLoS One. 2009 Nov 25;4(11):e8028. PMID:19946376<ref>PMID:19946376</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2wtl" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19946376}}, adds the Publication Abstract to the page
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*[[Ferritin 3D structures|Ferritin 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19946376 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19946376}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2WTL is a 6 chains structure of sequences from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WTL OCA].
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[[Category: Mycobacterium tuberculosis H37Rv]]
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[[Category: Gupta RK]]
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==Reference==
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[[Category: Gupta V]]
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<ref group="xtra">PMID:19946376</ref><references group="xtra"/>
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[[Category: Khare G]]
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[[Category: Mycobacterium tuberculosis]]
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[[Category: Salunke DM]]
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[[Category: Gupta, R K.]]
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[[Category: Tyagi AK]]
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[[Category: Gupta, V.]]
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[[Category: Khare, G.]]
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[[Category: Salunke, D M.]]
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[[Category: Tyagi, A K.]]
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[[Category: Bacterioferritin some]]
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[[Category: Biliverdin]]
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[[Category: Heme]]
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[[Category: Iron]]
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[[Category: Iron storage]]
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[[Category: Metal binding protein]]
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[[Category: Metal-binding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 16 13:01:46 2009''
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Current revision

Crystal structure of BfrA from M. tuberculosis

PDB ID 2wtl

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