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3kbh

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{{Seed}}
 
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[[Image:3kbh.jpg|left|200px]]
 
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<!--
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==Crystal structure of NL63 respiratory coronavirus receptor-binding domain complexed with its human receptor==
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The line below this paragraph, containing "STRUCTURE_3kbh", creates the "Structure Box" on the page.
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<StructureSection load='3kbh' size='340' side='right' caption='[[3kbh]], [[Resolution|resolution]] 3.31&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3kbh]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Cvhnl Cvhnl] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KBH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KBH FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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-->
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2ajf|2ajf]]</td></tr>
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{{STRUCTURE_3kbh| PDB=3kbh | SCENE= }}
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ACE2, spike protein, UNQ868/PRO1885 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), 2, human angiotensin-converting enzyme 2, S ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=277944 CVHNL])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kbh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kbh OCA], [http://pdbe.org/3kbh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3kbh RCSB], [http://www.ebi.ac.uk/pdbsum/3kbh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3kbh ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[http://www.uniprot.org/uniprot/ACE2_HUMAN ACE2_HUMAN]] Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.<ref>PMID:10969042</ref> <ref>PMID:10924499</ref> <ref>PMID:14647384</ref> [[http://www.uniprot.org/uniprot/SPIKE_CVHNL SPIKE_CVHNL]] S1 region attaches the virion to the cell membrane by interacting with human ACE2, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kb/3kbh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3kbh ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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NL63 coronavirus (NL63-CoV), a prevalent human respiratory virus, is the only group I coronavirus known to use angiotensin-converting enzyme 2 (ACE2) as its receptor. Incidentally, ACE2 is also used by group II SARS coronavirus (SARS-CoV). We investigated how different groups of coronaviruses recognize the same receptor, whereas homologous group I coronaviruses recognize different receptors. We determined the crystal structure of NL63-CoV spike protein receptor-binding domain (RBD) complexed with human ACE2. NL63-CoV RBD has a novel beta-sandwich core structure consisting of 2 layers of beta-sheets, presenting 3 discontinuous receptor-binding motifs (RBMs) to bind ACE2. NL63-CoV and SARS-CoV have no structural homology in RBD cores or RBMs; yet the 2 viruses recognize common ACE2 regions, largely because of a "virus-binding hotspot" on ACE2. Among group I coronaviruses, RBD cores are conserved but RBMs are variable, explaining how these viruses recognize different receptors. These results provide a structural basis for understanding viral evolution and virus-receptor interactions.
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===Crystal structure of NL63 respiratory coronavirus receptor-binding domain complexed with its human receptor===
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Crystal structure of NL63 respiratory coronavirus receptor-binding domain complexed with its human receptor.,Wu K, Li W, Peng G, Li F Proc Natl Acad Sci U S A. 2009 Nov 24;106(47):19970-4. Epub 2009 Nov 9. PMID:19901337<ref>PMID:19901337</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3kbh" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19901337}}, adds the Publication Abstract to the page
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*[[Angiotensin-Converting Enzyme|Angiotensin-Converting Enzyme]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19901337 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19901337}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Cvhnl]]
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3KBH is a 8 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Human_coronavirus_nl63 Human coronavirus nl63]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KBH OCA].
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[[Category: Human]]
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[[Category: Li, F]]
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==Reference==
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[[Category: Li, W]]
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<ref group="xtra">PMID:19901337</ref><references group="xtra"/>
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[[Category: Peng, G]]
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[[Category: Homo sapiens]]
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[[Category: Wu, K]]
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[[Category: Human coronavirus nl63]]
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[[Category: Li, F.]]
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[[Category: Li, W.]]
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[[Category: Peng, G.]]
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[[Category: Wu, K.]]
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[[Category: Alternative splicing]]
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[[Category: Beta sandwich]]
[[Category: Beta sandwich]]
[[Category: Carboxypeptidase]]
[[Category: Carboxypeptidase]]
[[Category: Cell membrane]]
[[Category: Cell membrane]]
[[Category: Chloride]]
[[Category: Chloride]]
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[[Category: Coiled coil]]
 
[[Category: Envelope protein]]
[[Category: Envelope protein]]
[[Category: Fusion protein]]
[[Category: Fusion protein]]
[[Category: Glycoprotein]]
[[Category: Glycoprotein]]
[[Category: Host-virus interaction]]
[[Category: Host-virus interaction]]
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[[Category: Hydrolase]]
[[Category: Membrane]]
[[Category: Membrane]]
[[Category: Metal-binding]]
[[Category: Metal-binding]]
[[Category: Metalloprotease]]
[[Category: Metalloprotease]]
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[[Category: Polymorphism]]
 
[[Category: Protease]]
[[Category: Protease]]
[[Category: Secreted]]
[[Category: Secreted]]
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[[Category: Virion]]
[[Category: Virion]]
[[Category: Virulence]]
[[Category: Virulence]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 16 13:02:38 2009''
 

Current revision

Crystal structure of NL63 respiratory coronavirus receptor-binding domain complexed with its human receptor

3kbh, resolution 3.31Å

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