3i06

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{{Seed}}
 
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[[Image:3i06.jpg|left|200px]]
 
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==Crystal structure of cruzain covalently bound to a purine nitrile==
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The line below this paragraph, containing "STRUCTURE_3i06", creates the "Structure Box" on the page.
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<StructureSection load='3i06' size='340' side='right'caption='[[3i06]], [[Resolution|resolution]] 1.10&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3i06]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I06 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I06 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=QL2:6-[(3,5-DIFLUOROPHENYL)AMINO]-9-ETHYL-9H-PURINE-2-CARBONITRILE'>QL2</scene></td></tr>
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{{STRUCTURE_3i06| PDB=3i06 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i06 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i06 OCA], [https://pdbe.org/3i06 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i06 RCSB], [https://www.ebi.ac.uk/pdbsum/3i06 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i06 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CYSP_TRYCR CYSP_TRYCR] Hydrolyzes chromogenic peptides at the carboxyl Arg or Lys; requires at least one more amino acid, preferably Arg, Phe, Val or Leu, between the terminal Arg or Lys and the amino-blocking group. The cysteine protease may play an important role in the development and differentiation of the parasites at several stages of their life cycle.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i0/3i06_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3i06 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Trypanosoma cruzi and Trypanosoma brucei are parasites that cause Chagas' disease and African sleeping sickness, respectively. Both parasites rely on essential cysteine proteases for survival: cruzain for T. cruzi and TbCatB/rhodesain for T. brucei. A recent quantitative high-throughput screen of cruzain identified triazine nitriles, which are known inhibitors of other cysteine proteases, as reversible inhibitors of the enzyme. Structural modifications detailed herein, including core scaffold modification from triazine to purine, improved the in vitro potency against both cruzain and rhodesain by 350-fold, while also gaining activity against T. brucei parasites. Selected compounds were screened against a panel of human cysteine and serine proteases to determine selectivity, and a cocrystal was obtained of our most potent analogue bound to cruzain.
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===Crystal structure of cruzain covalently bound to a purine nitrile===
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Identification and Optimization of Inhibitors of Trypanosomal Cysteine Proteases: Cruzain, Rhodesain, and TbCatB.,Mott BT, Ferreira RS, Simeonov A, Jadhav A, Ang KK, Leister W, Shen M, Silveira JT, Doyle PS, Arkin MR, McKerrow JH, Inglese J, Austin CP, Thomas CJ, Shoichet BK, Maloney DJ J Med Chem. 2009 Nov 12. PMID:19908842<ref>PMID:19908842</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3i06" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19908842}}, adds the Publication Abstract to the page
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*[[Cruzain|Cruzain]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19908842 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19908842}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3I06 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I06 OCA].
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==Reference==
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<ref group="xtra">PMID:19908842</ref><references group="xtra"/>
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[[Category: Cruzipain]]
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[[Category: Trypanosoma cruzi]]
[[Category: Trypanosoma cruzi]]
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[[Category: Ferreira, R S.]]
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[[Category: Ferreira RS]]
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[[Category: McKerrow, J H.]]
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[[Category: McKerrow JH]]
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[[Category: Shoichet, B K.]]
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[[Category: Shoichet BK]]
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[[Category: Autocatalytic cleavage]]
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[[Category: Glycoprotein]]
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[[Category: Protease]]
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[[Category: Thiol protease]]
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[[Category: Zymogen]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 16 13:30:43 2009''
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Current revision

Crystal structure of cruzain covalently bound to a purine nitrile

PDB ID 3i06

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