1yio

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(New page: 200px<br /><applet load="1yio" size="450" color="white" frame="true" align="right" spinBox="true" caption="1yio, resolution 2.20&Aring;" /> '''Crystallographic str...)
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[[Image:1yio.gif|left|200px]]<br /><applet load="1yio" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1yio, resolution 2.20&Aring;" />
 
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'''Crystallographic structure of response regulator StyR from Pseudomonas fluorescens'''<br />
 
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==Overview==
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==Crystallographic structure of response regulator StyR from Pseudomonas fluorescens==
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StyR belongs to the FixJ subfamily of signal transduction response, regulators; it controls transcription of the styABCD operon coding for, styrene catabolism in Pseudomonas fluorescens ST. The crystal structure of, unphosphorylated StyR is reported at 2.2 A resolution. StyR is composed of, an N-terminal regulatory domain (StyR-N) and a C-terminal DNA binding, domain (StyR-C). The two domains are separated by an elongated linker, alpha helix (34 residues), a new feature in known response regulator, structures. StyR-C is structured similarly to the DNA binding domain of, the response regulator NarL. StyR-N shows structural reorganization of the, phosphate receiving region involved in activation/homodimerization:, specific residues adopt an "active-like" conformation, and the alpha4, helix, involved in dimerization of the homologous FixJ response regulator, is trimmed to just one helical turn. Overall, structural considerations, suggest that phosphorylation may act as an allosteric switch, shifting a, preexisting StyR equilibrium toward the active, dimeric, DNA binding form.
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<StructureSection load='1yio' size='340' side='right'caption='[[1yio]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1yio]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YIO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YIO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yio OCA], [https://pdbe.org/1yio PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yio RCSB], [https://www.ebi.ac.uk/pdbsum/1yio PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yio ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/O30989_PSEFL O30989_PSEFL]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yi/1yio_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1yio ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1YIO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens] with HG and MG as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1YIO OCA].
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*[[Response regulator 3D structure|Response regulator 3D structure]]
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__TOC__
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==Reference==
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</StructureSection>
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An active-like structure in the unphosphorylated StyR response regulator suggests a phosphorylation- dependent allosteric activation mechanism., Milani M, Leoni L, Rampioni G, Zennaro E, Ascenzi P, Bolognesi M, Structure. 2005 Sep;13(9):1289-97. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16154086 16154086]
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[[Category: Large Structures]]
[[Category: Pseudomonas fluorescens]]
[[Category: Pseudomonas fluorescens]]
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[[Category: Single protein]]
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[[Category: Ascenzi P]]
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[[Category: Ascenzi, P.]]
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[[Category: Bolognesi M]]
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[[Category: Bolognesi, M.]]
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[[Category: Leoni L]]
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[[Category: Leoni, L.]]
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[[Category: Milani M]]
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[[Category: Milani, M.]]
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[[Category: Rampioni G]]
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[[Category: Rampioni, G.]]
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[[Category: Zennaro E]]
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[[Category: Zennaro, E.]]
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[[Category: HG]]
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[[Category: MG]]
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[[Category: transcription regulation]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 04:10:14 2007''
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Current revision

Crystallographic structure of response regulator StyR from Pseudomonas fluorescens

PDB ID 1yio

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