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3a9j

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{{Seed}}
 
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[[Image:3a9j.png|left|200px]]
 
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==Crystal structure of the mouse TAB2-NZF in complex with Lys63-linked di-ubiquitin==
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The line below this paragraph, containing "STRUCTURE_3a9j", creates the "Structure Box" on the page.
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<StructureSection load='3a9j' size='340' side='right'caption='[[3a9j]], [[Resolution|resolution]] 1.18&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3a9j]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A9J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3A9J FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3a9k|3a9k]]</div></td></tr>
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{{STRUCTURE_3a9j| PDB=3a9j | SCENE= }}
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Ubiquitin ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice]), TAB2 (AMINO ACIDS 665 - 693) ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3a9j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a9j OCA], [https://pdbe.org/3a9j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3a9j RCSB], [https://www.ebi.ac.uk/pdbsum/3a9j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3a9j ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/TAB2_MOUSE TAB2_MOUSE]] Adapter linking MAP3K7/TAK1 and TRAF6. Promotes MAP3K7 activation in the IL1 signaling pathway. The binding of 'Lys-63'-linked polyubiquitin chains to TAB2 promotes autophosphorylation of MAP3K7 at 'Thr-187' (By similarity). Regulates the IL1-mediated translocation of NCOR1 out of the nucleus. Involved in heart development (By similarity).<ref>PMID:12150997</ref> <ref>PMID:19927120</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a9/3a9j_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3a9j ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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TAB2 and TAB3 activate the Jun N-terminal kinase and nuclear factor-kappaB pathways through the specific recognition of Lys 63-linked polyubiquitin chains by its Npl4 zinc-finger (NZF) domain. Here we report crystal structures of the TAB2 and TAB3 NZF domains in complex with Lys 63-linked diubiquitin at 1.18 and 1.40 A resolutions, respectively. Both NZF domains bind to the distal ubiquitin through a conserved Thr-Phe dipeptide that has been shown to be important for the interaction of the NZF domain of Npl4 with monoubiquitin. In contrast, a surface specific to TAB2 and TAB3 binds the proximal ubiquitin. Both the distal and proximal binding sites of the TAB2 and TAB3 NZF domains recognize the Ile 44-centred hydrophobic patch on ubiquitin but do not interact with the Lys 63-linked isopeptide bond. Mutagenesis experiments show that both binding sites are required to enable binding of Lys 63-linked diubiquitin. We therefore propose a mechanism for the recognition of Lys 63-linked polyubiquitin chains by TAB2 and TAB3 NZF domains in which diubiquitin units are specifically recognized by a single NZF domain.
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===Crystal structure of the mouse TAB2-NZF in complex with Lys63-linked di-ubiquitin===
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Structural basis for specific recognition of Lys 63-linked polyubiquitin chains by NZF domains of TAB2 and TAB3.,Sato Y, Yoshikawa A, Yamashita M, Yamagata A, Fukai S EMBO J. 2009 Dec 16;28(24):3903-9. Epub . PMID:19927120<ref>PMID:19927120</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3a9j" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19927120}}, adds the Publication Abstract to the page
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*[[3D structures of ubiquitin|3D structures of ubiquitin]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19927120 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19927120}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3A9J is a 3 chains structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A9J OCA].
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[[Category: Lk3 transgenic mice]]
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[[Category: Fukai, S]]
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==Reference==
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[[Category: Sato, Y]]
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<ref group="xtra">PMID:19927120</ref><references group="xtra"/>
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[[Category: Yamagata, A]]
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[[Category: Mus musculus]]
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[[Category: Yamashita, M]]
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[[Category: Fukai, S.]]
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[[Category: Yoshikawa, A]]
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[[Category: Sato, Y.]]
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[[Category: Yamagata, A.]]
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[[Category: Yamashita, M.]]
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[[Category: Yoshikawa, A.]]
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[[Category: Cytoplasm]]
[[Category: Cytoplasm]]
[[Category: Isopeptide bond]]
[[Category: Isopeptide bond]]
[[Category: Metal-binding]]
[[Category: Metal-binding]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: Signaling protein-metal binding protein complex]]
[[Category: Zinc]]
[[Category: Zinc]]
[[Category: Zinc-finger]]
[[Category: Zinc-finger]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 23 08:59:54 2009''
 

Current revision

Crystal structure of the mouse TAB2-NZF in complex with Lys63-linked di-ubiquitin

PDB ID 3a9j

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