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3ksl

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{{Seed}}
 
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[[Image:3ksl.jpg|left|200px]]
 
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==Structure of FPT bound to DATFP-DH-GPP==
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The line below this paragraph, containing "STRUCTURE_3ksl", creates the "Structure Box" on the page.
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<StructureSection load='3ksl' size='340' side='right'caption='[[3ksl]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3ksl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KSL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KSL FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SZH:(2S,6E)-8-{[(R)-HYDROXY(PHOSPHONOOXY)PHOSPHORYL]OXY}-2,6-DIMETHYLOCT-6-EN-1-YL+(2S)-3,3,3-TRIFLUORO-2-HYDRAZINOPROPANOATE'>SZH</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_3ksl| PDB=3ksl | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ksl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ksl OCA], [https://pdbe.org/3ksl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ksl RCSB], [https://www.ebi.ac.uk/pdbsum/3ksl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ksl ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FNTA_RAT FNTA_RAT] Catalyzes the transfer of a farnesyl or geranyl-geranyl moiety from farnesyl or geranyl-geranyl pyrophosphate to a cysteine at the fourth position from the C-terminus of several proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X. The alpha subunit is thought to participate in a stable complex with the substrate. The beta subunit binds the peptide substrate. Through RAC1 prenylation and activation may positively regulate neuromuscular junction development downstream of MUSK (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ks/3ksl_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ksl ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Photoactive analogs of farnesyl diphosphate (FPP) are useful probes in studies of enzymes that employ this molecule as a substrate. Here, we describe the preparation and properties of two new FPP analogs that contain diazotrifluoropropanoyl photophores linked to geranyl diphosphate via amide or ester linkages. The amide-linked analog (3) was synthesized in 32P-labeled form from geraniol in seven steps. Experiments with Saccharomyces cerevisiae protein farnesyltransferase (ScPFTase) showed that 3 is an alternative substrate for the enzyme. Photolysis experiments with [(32)P]3 demonstrate that this compound labels the beta-subunits of both farnesyltransferase and geranylgeranyltransferase (types 1 and 2). However, the amide-linked probe 3 undergoes a rearrangement to a photochemically unreactive isomeric triazolone upon long term storage making it inconvenient to use. To address this stability issue, the ester-linked analog 4 was prepared in six steps from geraniol. Computational analysis and X-ray crystallographic studies suggest that 4 binds to protein farnesyl transferase (PFTase) in a similar fashion as FPP. Compound 4 is also an alternative substrate for PFTase, and a 32P-labeled form selectively photocrosslinks the beta-subunit of ScPFTase as well as E. coli farnesyldiphosphate synthase and a germacrene-producing sesquiterpene synthase from Nostoc sp. strain PCC7120 (a cyanobacterial source). Finally, nearly exclusive labeling of ScPFTase in crude E. coli extract was observed, suggesting that [32P]4 manifests significant selectivity and should hence be useful for identifying novel FPP-utilizing enzymes in crude protein preparations.
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===Structure of FPT bound to DATFP-DH-GPP===
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Synthesis, properties, and applications of diazotrifluropropanoyl-containing photoactive analogs of farnesyl diphosphate containing modified linkages for enhanced stability.,Hovlid ML, Edelstein RL, Henry O, Ochocki J, DeGraw A, Lenevich S, Talbot T, Young VG, Hruza AW, Lopez-Gallego F, Labello NP, Strickland CL, Schmidt-Dannert C, Distefano MD Chem Biol Drug Des. 2010 Jan;75(1):51-67. PMID:19954434<ref>PMID:19954434</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3ksl" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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3KSL is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KSL OCA].
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*[[Farnesyltransferase 3D structures|Farnesyltransferase 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:19954434</ref><references group="xtra"/>
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__TOC__
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[[Category: Protein farnesyltransferase]]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: DeGraw, A.]]
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[[Category: DeGraw A]]
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[[Category: Distefano, M D.]]
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[[Category: Distefano MD]]
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[[Category: Edelstein, R L.]]
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[[Category: Edelstein RL]]
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[[Category: Henry, O.]]
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[[Category: Henry O]]
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[[Category: Hovlid, M L.]]
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[[Category: Hovlid ML]]
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[[Category: Hruza, A W.]]
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[[Category: Hruza AW]]
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[[Category: Labello, N P.]]
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[[Category: Labello NP]]
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[[Category: Lenevich, S.]]
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[[Category: Lenevich S]]
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[[Category: Lopez-Gallego, F.]]
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[[Category: Lopez-Gallego F]]
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[[Category: Ochocki, J.]]
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[[Category: Ochocki J]]
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[[Category: Schmidt-Dannert, C.]]
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[[Category: Schmidt-Dannert C]]
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[[Category: Strickland, C L.]]
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[[Category: Strickland CL]]
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[[Category: Talbot, T.]]
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[[Category: Talbot T]]
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[[Category: Young, V.]]
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[[Category: Young V]]
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[[Category: Metal-binding]]
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[[Category: Phosphoprotein]]
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[[Category: Prenyltransferase]]
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[[Category: Transferase]]
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[[Category: Zinc]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 23 09:55:10 2009''
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Current revision

Structure of FPT bound to DATFP-DH-GPP

PDB ID 3ksl

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