This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1ynm

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1ynm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ynm, resolution 2.65&Aring;" /> '''Crystal structure of...)
Current revision (08:58, 14 February 2024) (edit) (undo)
 
(14 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1ynm.gif|left|200px]]<br /><applet load="1ynm" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1ynm, resolution 2.65&Aring;" />
 
-
'''Crystal structure of restriction endonuclease HinP1I'''<br />
 
-
==Overview==
+
==Crystal structure of restriction endonuclease HinP1I==
-
HinP1I, a type II restriction endonuclease, recognizes and cleaves a, palindromic tetranucleotide sequence (G/CGC) in double-stranded DNA, producing 2 nt 5' overhanging ends. Here, we report the structure of, HinP1I crystallized as one protein monomer in the crystallographic, asymmetric unit. HinP1I displays an elongated shape, with a conserved, catalytic core domain containing an active-site motif of SDX18QXK and a, putative DNA-binding domain. Without significant sequence homology, HinP1I, displays striking structural similarity to MspI, an endonuclease that, cleaves a similar palindromic DNA sequence (C/CGG) and binds to that, sequence crystallographically as a monomer. Almost all the structural, elements of MspI can be matched in HinP1I, including both the DNA, recognition and catalytic elements. Examining the protein-protein, interactions in the crystal lattice, HinP1I could be dimerized through two, helices located on the opposite side of the protein to the active site, generating a molecule with two active sites and two DNA-binding surfaces, opposite one another on the outer surfaces of the dimer. A possible, functional link between this unusual dimerization mode and the tetrameric, restriction enzymes is discussed.
+
<StructureSection load='1ynm' size='340' side='right'caption='[[1ynm]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1ynm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YNM FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ynm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ynm OCA], [https://pdbe.org/1ynm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ynm RCSB], [https://www.ebi.ac.uk/pdbsum/1ynm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ynm ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/Q5I6E6_HAEIF Q5I6E6_HAEIF]
-
==About this Structure==
+
==See Also==
-
1YNM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Active as [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1YNM OCA].
+
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Structure of HinP1I endonuclease reveals a striking similarity to the monomeric restriction enzyme MspI., Yang Z, Horton JR, Maunus R, Wilson GG, Roberts RJ, Cheng X, Nucleic Acids Res. 2005 Apr 1;33(6):1892-901. Print 2005. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15805123 15805123]
+
[[Category: Haemophilus influenzae]]
[[Category: Haemophilus influenzae]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Type II site-specific deoxyribonuclease]]
+
[[Category: Cheng X]]
-
[[Category: Cheng, X.]]
+
[[Category: Horton JR]]
-
[[Category: Horton, J.R.]]
+
[[Category: Maunus R]]
-
[[Category: Maunus, R.]]
+
[[Category: Roberts RJ]]
-
[[Category: Roberts, R.J.]]
+
[[Category: Wilson GG]]
-
[[Category: Wilson, G.G.]]
+
[[Category: Yang Z]]
-
[[Category: Yang, Z.]]
+
-
[[Category: dimerizaton]]
+
-
[[Category: restriction endonuclease]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 04:24:56 2007''
+

Current revision

Crystal structure of restriction endonuclease HinP1I

PDB ID 1ynm

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ynm"
Personal tools