3go8

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{{Seed}}
 
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[[Image:3go8.png|left|200px]]
 
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==MutM encountering an intrahelical 8-oxoguanine (oxoG) lesion in EC3-loop deletion complex==
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The line below this paragraph, containing "STRUCTURE_3go8", creates the "Structure Box" on the page.
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<StructureSection load='3go8' size='340' side='right'caption='[[3go8]], [[Resolution|resolution]] 1.89&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3go8]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GO8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GO8 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.89&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=8OG:8-OXO-2-DEOXY-GUANOSINE-5-MONOPHOSPHATE'>8OG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_3go8| PDB=3go8 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3go8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3go8 OCA], [https://pdbe.org/3go8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3go8 RCSB], [https://www.ebi.ac.uk/pdbsum/3go8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3go8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/P84131_GEOSE P84131_GEOSE] Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates (By similarity).[HAMAP-Rule:MF_00103][SAAS:SAAS020629_004_120556]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/go/3go8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3go8 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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How living systems detect the presence of genotoxic damage embedded in a million-fold excess of undamaged DNA is an unresolved question in biology. Here we have captured and structurally elucidated a base-excision DNA repair enzyme, MutM, at the stage of initial encounter with a damaged nucleobase, 8-oxoguanine (oxoG), nested within a DNA duplex. Three structures of intrahelical oxoG-encounter complexes are compared with sequence-matched structures containing a normal G base in place of an oxoG lesion. Although the protein-DNA interfaces in the matched complexes differ by only two atoms-those that distinguish oxoG from G-their pronounced structural differences indicate that MutM can detect a lesion in DNA even at the earliest stages of encounter. All-atom computer simulations show the pathway by which encounter of the enzyme with the lesion causes extrusion from the DNA duplex, and they elucidate the critical free energy difference between oxoG and G along the extrusion pathway.
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===MutM encountering an intrahelical 8-oxoguanine (oxoG) lesion in EC3-loop deletion complex===
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Encounter and extrusion of an intrahelical lesion by a DNA repair enzyme.,Qi Y, Spong MC, Nam K, Banerjee A, Jiralerspong S, Karplus M, Verdine GL Nature. 2009 Dec 10;462(7274):762-6. PMID:20010681<ref>PMID:20010681</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3go8" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_20010681}}, adds the Publication Abstract to the page
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*[[CD1|CD1]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20010681 is the PubMed ID number.
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*[[DNA glycosylase 3D structures|DNA glycosylase 3D structures]]
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== References ==
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{{ABSTRACT_PUBMED_20010681}}
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<references/>
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__TOC__
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==About this Structure==
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</StructureSection>
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3GO8 is a 3 chains structure of sequences from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GO8 OCA].
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==Reference==
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<ref group="xtra">PMID:20010681</ref><references group="xtra"/>
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[[Category: Geobacillus stearothermophilus]]
[[Category: Geobacillus stearothermophilus]]
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[[Category: Qi, Y.]]
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[[Category: Large Structures]]
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[[Category: Spong, M C.]]
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[[Category: Synthetic construct]]
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[[Category: Verdine, G L.]]
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[[Category: Qi Y]]
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[[Category: Base extrusion]]
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[[Category: Spong MC]]
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[[Category: Damage search]]
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[[Category: Verdine GL]]
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[[Category: Disulfide crosslinking]]
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[[Category: Dna damage]]
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[[Category: Dna glycosylase]]
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[[Category: Dna repair]]
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[[Category: Dna-binding]]
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[[Category: Glycosidase]]
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[[Category: Hydrolase]]
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[[Category: Lyase]]
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[[Category: Lyase/dna complex]]
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[[Category: Metal-binding]]
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[[Category: Multifunctional enzyme]]
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[[Category: Zinc]]
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[[Category: Zinc-finger]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 30 13:59:26 2009''
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Current revision

MutM encountering an intrahelical 8-oxoguanine (oxoG) lesion in EC3-loop deletion complex

PDB ID 3go8

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