1qwb

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(New page: 200px<br /><applet load="1qwb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qwb" /> '''NMR strucutre of 5'-r(GGACACGAAAUCCCGAAGUAGU...)
Current revision (06:07, 17 April 2024) (edit) (undo)
 
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[[Image:1qwb.gif|left|200px]]<br /><applet load="1qwb" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1qwb" />
 
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'''NMR strucutre of 5'-r(GGACACGAAAUCCCGAAGUAGUGUCC)-3' : an RNA hairpin containing the in vitro selected consensus sequence for nucleolin RBD12'''<br />
 
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==Overview==
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==NMR structure of 5'-r(GGACACGAAAUCCCGAAGUAGUGUCC)-3' : an RNA hairpin containing the in vitro selected consensus sequence for nucleolin RBD12==
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Nucleolin, a multi-domain protein involved in ribosome biogenesis, has, been shown to bind the consensus sequence (U/G)CCCG(A/G) in the context of, a hairpin loop structure (nucleolin recognition element; NRE). Previous, studies have shown that the first two RNA-binding domains in nucleolin, (RBD12) are responsible for the interaction with the in vitro selected NRE, (sNRE). We have previously reported the structures of nucleolin RBD12, sNRE and nucleolin RBD12-sNRE complex. A comparison of free and bound sNRE, shows that the NRE loop becomes structured upon binding. From this, observation, we hypothesized that the disordered hairpin loop of sNRE, facilitates conformational rearrangements when the protein binds. Here, we, show that nucleolin RBD12 is also sufficient for sequence- specific, binding of two NRE sequences found in pre-rRNA, b1NRE and b2NRE., Structural investigations of the free NREs using NMR spectroscopy show, that the b1NRE loop is conformationally heterogeneous, while the b2NRE, loop is structured. The b2NRE forms a hairpin capped by a YNMG-like, tetraloop. Comparison of the chemical shifts of sNRE and b2NRE in complex, with nucleolin RBD12 suggests that the NRE consensus nucleotides adopt a, similar conformation. These results show that a disordered NRE consensus, sequence is not a prerequisite for nucleolin RBD12 binding.
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<StructureSection load='1qwb' size='340' side='right'caption='[[1qwb]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1qwb]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QWB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QWB FirstGlance]. <br>
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1QWB is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QWB OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qwb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qwb OCA], [https://pdbe.org/1qwb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qwb RCSB], [https://www.ebi.ac.uk/pdbsum/1qwb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qwb ProSAT]</span></td></tr>
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==Reference==
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</table>
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Solution structures of stem-loop RNAs that bind to the two N-terminal RNA-binding domains of nucleolin., Finger LD, Trantirek L, Johansson C, Feigon J, Nucleic Acids Res. 2003 Nov 15;31(22):6461-72. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14602904 14602904]
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__TOC__
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[[Category: Protein complex]]
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</StructureSection>
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[[Category: Feigon, J.]]
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[[Category: Large Structures]]
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[[Category: Finger, L.D.]]
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[[Category: Feigon J]]
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[[Category: Johansson, C.]]
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[[Category: Finger LD]]
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[[Category: Trantirek, L.]]
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[[Category: Johansson C]]
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[[Category: a-form helix]]
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[[Category: Trantirek L]]
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[[Category: disordered hairpin loop]]
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[[Category: loop e motif]]
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[[Category: s-turn]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 04:29:13 2007''
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Current revision

NMR structure of 5'-r(GGACACGAAAUCCCGAAGUAGUGUCC)-3' : an RNA hairpin containing the in vitro selected consensus sequence for nucleolin RBD12

PDB ID 1qwb

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