3fo9

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{{Seed}}
 
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[[Image:3fo9.png|left|200px]]
 
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==Crystal structure of aldolase antibody 33F12 Fab' in complex with hapten 1,3-diketone==
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The line below this paragraph, containing "STRUCTURE_3fo9", creates the "Structure Box" on the page.
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<StructureSection load='3fo9' size='340' side='right'caption='[[3fo9]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3fo9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FO9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FO9 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DIK:5-{[4-(5-METHYL-3-OXOHEX-4-EN-1-YL)PHENYL]AMINO}-5-OXOPENTANOIC+ACID'>DIK</scene></td></tr>
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{{STRUCTURE_3fo9| PDB=3fo9 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fo9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fo9 OCA], [https://pdbe.org/3fo9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fo9 RCSB], [https://www.ebi.ac.uk/pdbsum/3fo9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fo9 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GCAM_MOUSE GCAM_MOUSE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fo/3fo9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fo9 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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An enamine intermediate is believed to be the central feature of biological catalysts, such as aldolases and small molecule amine organocatalysts. Despite decades of investigation of naturally occurring aldolase enzymes and recent studies on designed aldolase antibodies and organocatalysts, direct structural observation of an enamine intermediate has proven to be rare. Herein, we report the observation of a stable enamine intermediate in the crystal structure of an aldolase antibody 33F12 in complex with a 1,3-diketone derivative. This enamine complex structure provides strong evidence that fewer residues are essential for amine catalysis within the hydrophobic environments of this catalytic antibody than speculated for natural aldolase enzymes and should serve to guide future studies aimed at the rational design of these types of catalysts, as well as organocatalysts. Indeed, enamine catalysis in proteins might be more simplistic than previously imagined.
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===Crystal structure of aldolase antibody 33F12 Fab' in complex with hapten 1,3-diketone===
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Direct observation of an enamine intermediate in amine catalysis.,Zhu X, Tanaka F, Lerner RA, Barbas Iii CF, Wilson IA J Am Chem Soc. 2009 Dec 30;131(51):18206-7. PMID:19968282<ref>PMID:19968282</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19968282}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3fo9" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19968282 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19968282}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3FO9 is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FO9 OCA].
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==Reference==
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<ref group="xtra">PMID:19968282</ref><references group="xtra"/>
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[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Wilson, I A.]]
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[[Category: Wilson IA]]
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[[Category: Zhu, X.]]
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[[Category: Zhu X]]
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[[Category: Aldolase antibody]]
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[[Category: Amine catalysis]]
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[[Category: Enamine intermediate]]
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[[Category: Immune system]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 13 13:25:20 2010''
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Current revision

Crystal structure of aldolase antibody 33F12 Fab' in complex with hapten 1,3-diketone

PDB ID 3fo9

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