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3acp
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 3acp is ON HOLD Authors: Sangwoo K., Yasushi S., Atsuo S., Kenji T., Keisuke F., Takashi Y., Koichi K., Keiji T., Tsunehiro M. Description: Crystal...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal Structure of Yeast Rpn14, a Chaperone of the 19S Regulatory Particle of the Proteasome== | |
| + | <StructureSection load='3acp' size='340' side='right'caption='[[3acp]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3acp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ACP OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3ACP FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3acp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3acp OCA], [http://pdbe.org/3acp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3acp RCSB], [http://www.ebi.ac.uk/pdbsum/3acp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3acp ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ac/3acp_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3acp ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The ubiquitin-proteasome pathway is a major proteolytic system in eukaryotic cells and regulates various cellular processes. The 26 S proteasome, the central enzyme of this pathway, consists of a proteolytic core particle and two 19 S regulatory particles (RPs) composed of ATPase (Rpt) and non-ATPase (Rpn) subunits. Growing evidence indicates that proteasome assembly is assisted by a variety of chaperones. In particular, it has been reported recently that Nas2, Nas6, Rpn14, and Hsm3 bind specific Rpt subunits, thereby contributing to the formation of 19 S RP. Rpn14 transiently binds to the C-terminal domain of the Rpt6 subunit (Rpt6-C) during maturation of the ATPase ring of 19 S RP. In this study, we determined the crystal structure of yeast Rpn14 at 2.0 A resolution, which revealed that this chaperone consists of a unique N-terminal domain with unknown function and a C-terminal domain assuming a canonical seven-bladed beta-propeller fold. The Rpt6-binding site on Rpn14 was predicted based on structural comparison with the complex formed between Nas6 and Rpt3-C. The top face of Rpn14 exhibits a highly acidic surface area, whereas the putative interacting surface of Rpt6-C is basic. By inspection of structural data along with genetic and biochemical data, we determined the specific residues of Rpn14 and Rpt6 for complementary charge interactions that are required for 19 S RP assembly. | ||
| - | + | Crystal structure of yeast rpn14, a chaperone of the 19 S regulatory particle of the proteasome.,Kim S, Saeki Y, Fukunaga K, Suzuki A, Takagi K, Yamane T, Tanaka K, Mizushima T, Kato K J Biol Chem. 2010 May 14;285(20):15159-66. Epub 2010 Mar 16. PMID:20236927<ref>PMID:20236927</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 3acp" style="background-color:#fffaf0;"></div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Atcc 18824]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Fukunaga, K]] | ||
| + | [[Category: Kato, K]] | ||
| + | [[Category: Kim, S]] | ||
| + | [[Category: Mizushima, T]] | ||
| + | [[Category: Saeki, Y]] | ||
| + | [[Category: Suzuki, A]] | ||
| + | [[Category: Takagi, K]] | ||
| + | [[Category: Tanaka, K]] | ||
| + | [[Category: Yamane, T]] | ||
| + | [[Category: Chaperone]] | ||
| + | [[Category: Wd repeat]] | ||
| + | [[Category: Wd40 domain]] | ||
Current revision
Crystal Structure of Yeast Rpn14, a Chaperone of the 19S Regulatory Particle of the Proteasome
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Categories: Atcc 18824 | Large Structures | Fukunaga, K | Kato, K | Kim, S | Mizushima, T | Saeki, Y | Suzuki, A | Takagi, K | Tanaka, K | Yamane, T | Chaperone | Wd repeat | Wd40 domain
