3hi2

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[[Image:3hi2.jpg|left|200px]]
 
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==Structure of the N-terminal domain of the E. coli antitoxin MqsA (YgiT/b3021) in complex with the E. coli toxin MqsR (YgiU/b3022)==
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The line below this paragraph, containing "STRUCTURE_3hi2", creates the "Structure Box" on the page.
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<StructureSection load='3hi2' size='340' side='right'caption='[[3hi2]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3hi2]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HI2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HI2 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_3hi2| PDB=3hi2 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hi2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hi2 OCA], [https://pdbe.org/3hi2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hi2 RCSB], [https://www.ebi.ac.uk/pdbsum/3hi2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hi2 ProSAT]</span></td></tr>
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</table>
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===Structure of the N-terminal domain of the E. coli antitoxin MqsA (YgiT/b3021) in complex with the E. coli toxin MqsR (YgiU/b3022)===
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== Function ==
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[https://www.uniprot.org/uniprot/MQSA_ECOLI MQSA_ECOLI] Antitoxin component of a type II toxin-antitoxin (TA) module. Labile antitoxin that binds to the MqsR mRNA interferase toxin and neutralizes its endoribonuclease activity. Overexpression prevents MqsR-mediated cessation of cell growth and inhibition of cell proliferation. Initially reported to act as a cotranscription factor with MqsA (PubMed:19690171, PubMed:20105222). Following further experiments, the MqsR-MqsA complex does not bind DNA and all reported data are actually due to a small fraction of free MqsA alone binding DNA. Addition of MqsR to a preformed MqsA-promoter DNA complex causes dissociation of the MqsA-DNA complex, probably causing derepression of MqsA-repressed transcripts (PubMed:23172222). MqsA binds to 2 palindromes in the promoter region of the mqsRA operon activating its transcription. Binds to other promoters, inducing mcbR and spy and repressing cspD among others (PubMed:20105222). Binds to and represses the rpoS promoter, the master stress regulator, resulting in decreased cyclic-di-GMP, reduced stress resistance, increased cell motility and decreased biofilm formation; in these experiments 5 TA modules are missing (lacks MazEF, RelEB, ChpB, YoeB-YefM, YafQ-DinJ) (PubMed:21516113). An earlier study showed overexpression alone increases biofilm formation, perhaps by repressing cspD; in these experiments the 5 TA modules are present (PubMed:20105222). Represses the csgD promoter. In the presence of stress, when this protein is degraded, the promoters it represses are derepressed, leading to biofilm formation (Probable). This TA system mediates cell growth during bile acid deoxycholate stress by degrading mRNA for probable deoxycholate-binding protein YgiS; bile acid detergents such as deoxycholate are important for host defense against bacterial growth in the gall bladder and duodenum (PubMed:25534751).<ref>PMID:19690171</ref> <ref>PMID:19943910</ref> <ref>PMID:20105222</ref> <ref>PMID:21516113</ref> <ref>PMID:23172222</ref> <ref>PMID:25534751</ref> <ref>PMID:24212724</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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(as it appears on PubMed at http://www.pubmed.gov), where 20041169 is the PubMed ID number.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hi/3hi2_consurf.spt"</scriptWhenChecked>
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{{ABSTRACT_PUBMED_20041169}}
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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3HI2 is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HI2 OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hi2 ConSurf].
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<div style="clear:both"></div>
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==Reference==
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== References ==
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<ref group="xtra">PMID:20041169</ref><references group="xtra"/>
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<references/>
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[[Category: Escherichia coli k-12]]
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__TOC__
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[[Category: Arruda, J M.]]
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</StructureSection>
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[[Category: Brown, B L.]]
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[[Category: Escherichia coli K-12]]
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[[Category: Grigoriu, S.]]
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[[Category: Large Structures]]
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[[Category: Page, R.]]
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[[Category: Arruda JM]]
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[[Category: Peti, W.]]
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[[Category: Brown BL]]
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[[Category: B3021]]
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[[Category: Grigoriu S]]
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[[Category: B3022]]
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[[Category: Page R]]
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[[Category: Mqsa]]
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[[Category: Peti W]]
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[[Category: Mqsr]]
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[[Category: Quorum sensing]]
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[[Category: Stress response]]
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[[Category: Toxin-antitoxin system]]
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[[Category: Ygit]]
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[[Category: Ygiu]]
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[[Category: Zn-binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 13 13:47:41 2010''
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Current revision

Structure of the N-terminal domain of the E. coli antitoxin MqsA (YgiT/b3021) in complex with the E. coli toxin MqsR (YgiU/b3022)

PDB ID 3hi2

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