3ks8

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{{Seed}}
 
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[[Image:3ks8.jpg|left|200px]]
 
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==Crystal structure of Reston ebolavirus VP35 RNA binding domain in complex with 18bp dsRNA==
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The line below this paragraph, containing "STRUCTURE_3ks8", creates the "Structure Box" on the page.
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<StructureSection load='3ks8' size='340' side='right'caption='[[3ks8]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3ks8]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Reston_ebolavirus_-_Reston Reston ebolavirus - Reston]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KS8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KS8 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.401&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ks8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ks8 OCA], [https://pdbe.org/3ks8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ks8 RCSB], [https://www.ebi.ac.uk/pdbsum/3ks8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ks8 ProSAT]</span></td></tr>
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{{STRUCTURE_3ks8| PDB=3ks8 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/VP35_EBORR VP35_EBORR] Acts as a polymerase cofactor in the RNA polymerase transcription and replication complex. Prevents establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of interferon regulatory factor 3 (IRF3), a transcription factor critical for the induction of interferons alpha and beta. The mechanism by which this blockage occurs remains incompletely defined, a hypothesis suggests that VP35 dsRNA-binding activity prevents activation of IRF3 by sequestering dsRNA. Also inhibits the antiviral effect mediated by the interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ks/3ks8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ks8 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Ebolavirus causes a severe hemorrhagic fever and is divided into five distinct species, of which Reston ebolavirus is uniquely nonpathogenic to humans. Disease caused by ebolavirus is marked by early immunosuppression of innate immune signaling events, involving silencing and sequestration of double-stranded RNA (dsRNA) by the viral protein VP35. Here we present unbound and dsRNA-bound crystal structures of the dsRNA-binding domain of Reston ebolavirus VP35. The structures show that VP35 forms an unusual, asymmetric dimer on dsRNA binding, with each of the monomers binding dsRNA in a different way: one binds the backbone whereas the other caps the terminus. Additional SAXS, DXMS, and dsRNA-binding experiments presented here support a model of cooperative dsRNA recognition in which binding of the first monomer assists binding of the next monomer of the oligomeric VP35 protein. This work illustrates how ebolavirus VP35 could mask key recognition sites of molecules such as RIG-I, MDA-5, and Dicer to silence viral dsRNA in infection.
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===Crystal structure of Reston ebolavirus VP35 RNA binding domain in complex with 18bp dsRNA===
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Ebolavirus VP35 uses a bimodal strategy to bind dsRNA for innate immune suppression.,Kimberlin CR, Bornholdt ZA, Li S, Woods VL Jr, Macrae IJ, Saphire EO Proc Natl Acad Sci U S A. 2009 Dec 14. PMID:20018665<ref>PMID:20018665</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20018665}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3ks8" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20018665 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20018665}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3KS8 is a 6 chains structure of sequences from [http://en.wikipedia.org/wiki/Reston_ebolavirus Reston ebolavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KS8 OCA].
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[[Category: Reston ebolavirus - Reston]]
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[[Category: Bornholdt ZA]]
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==Reference==
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[[Category: Kimberlin CR]]
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<ref group="xtra">PMID:20018665</ref><references group="xtra"/>
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[[Category: Li S]]
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[[Category: Reston ebolavirus]]
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[[Category: Macrae IJ]]
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[[Category: Bornholdt, Z A.]]
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[[Category: Saphire EO]]
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[[Category: Kimberlin, C R.]]
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[[Category: Woods VL]]
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[[Category: Li, S.]]
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[[Category: Macrae, I J.]]
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[[Category: Saphire, E O.]]
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[[Category: Woods, V L.]]
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[[Category: Ebolavirus]]
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[[Category: Host cytoplasm]]
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[[Category: Interferon]]
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[[Category: Interferon antiviral system evasion]]
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[[Category: Protein-rna complex]]
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[[Category: Reston]]
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[[Category: Rna replication]]
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[[Category: Rna-binding]]
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[[Category: Rna-binding protein]]
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[[Category: Transcription]]
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[[Category: Virion]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 13 14:04:16 2010''
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Current revision

Crystal structure of Reston ebolavirus VP35 RNA binding domain in complex with 18bp dsRNA

PDB ID 3ks8

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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