3hxs

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{{Seed}}
 
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[[Image:3hxs.jpg|left|200px]]
 
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==Crystal Structure of Bacteroides fragilis TrxP==
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The line below this paragraph, containing "STRUCTURE_3hxs", creates the "Structure Box" on the page.
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<StructureSection load='3hxs' size='340' side='right'caption='[[3hxs]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3hxs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteroides_fragilis Bacteroides fragilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HXS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HXS FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.996&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_3hxs| PDB=3hxs | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hxs OCA], [https://pdbe.org/3hxs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hxs RCSB], [https://www.ebi.ac.uk/pdbsum/3hxs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hxs ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q64SV7_BACFR Q64SV7_BACFR]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hx/3hxs_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hxs ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Current dogma dictates that bacterial proteins with misoxidized disulfide bonds are shuffled into correctly oxidized states by DsbC. There are two proposed mechanisms for DsbC activity. The first involves a DsbC-only model of substrate disulfide rearrangement. The second invokes cycles of reduction and oxidation of substrate disulfide bonds by DsbC and DsbA respectively. Here, we addressed whether the second mechanism is important in vivo by identifying whether a periplasmic reductase could complement DsbC. We screened for naturally occurring periplasmic reductases in Bacteroides fragilis, a bacterium chosen because we predicted it encodes reductases and has a reducing periplasm. We found that the B. fragilis periplasmic protein TrxP has a thioredoxin fold with an extended N-terminal region; that it is a very active reductase but a poor isomerase; and that it fully complements dsbC. These results provide direct in vivo evidence that correctly folded protein is achievable via cycles of oxidation and reduction.
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===Crystal Structure of Bacteroides fragilis TrxP===
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In vivo oxidative protein folding can be facilitated by oxidation-reduction cycling.,Shouldice SR, Cho SH, Boyd D, Heras B, Eser M, Beckwith J, Riggs P, Martin JL, Berkmen M Mol Microbiol. 2010 Jan;75(1):13-28. Epub 2009 Dec 3. PMID:19968787<ref>PMID:19968787</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3hxs" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19968787}}, adds the Publication Abstract to the page
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*[[Thioredoxin 3D structures|Thioredoxin 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19968787 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19968787}}
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__TOC__
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</StructureSection>
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==About this Structure==
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3HXS is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Bacteroides_fragilis Bacteroides fragilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HXS OCA].
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==Reference==
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<ref group="xtra">PMID:19968787</ref><references group="xtra"/>
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[[Category: Bacteroides fragilis]]
[[Category: Bacteroides fragilis]]
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[[Category: Shouldice, S R.]]
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[[Category: Large Structures]]
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[[Category: Electron transport]]
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[[Category: Shouldice SR]]
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[[Category: Thioredoxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 13 14:14:23 2010''
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Current revision

Crystal Structure of Bacteroides fragilis TrxP

PDB ID 3hxs

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