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3a58

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{{Seed}}
 
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[[Image:3a58.jpg|left|200px]]
 
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==Crystal structure of Sec3p - Rho1p complex from Saccharomyces cerevisiae==
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The line below this paragraph, containing "STRUCTURE_3a58", creates the "Structure Box" on the page.
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<StructureSection load='3a58' size='340' side='right'caption='[[3a58]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3a58]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A58 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3A58 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_3a58| PDB=3a58 | SCENE= }}
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SEC3, PSL1, YER008C ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), RHO1, YPR165W, P9325.3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3a58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a58 OCA], [https://pdbe.org/3a58 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3a58 RCSB], [https://www.ebi.ac.uk/pdbsum/3a58 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3a58 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/SEC3_YEAST SEC3_YEAST]] Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. [[https://www.uniprot.org/uniprot/RHO1_YEAST RHO1_YEAST]] Acts as a central regulator in the cell wall integrity signaling pathway, which is regulated by the cell cycle and in response to various types of cell wall stress. Integrates signals from different cell surface sensors, and activates a set of effectors, regulating processes including beta-glucan synthesis at the site of wall remodeling, gene expression related to cell wall biogenesis, organization of the actin cytoskeleton, and protein- and secretory vesicle-targeting to the growth site. Activates the protein kinase C (PKC1) MAP kinase cascade, the beta-1,3-glucan synthase (FKS1), the formin BNI1, the exocyst component SEC3 and the transcription factor SKN7.<ref>PMID:8195291</ref> <ref>PMID:7579704</ref> <ref>PMID:8846785</ref> <ref>PMID:8947028</ref> <ref>PMID:8621575</ref> <ref>PMID:8602514</ref> <ref>PMID:8602515</ref> <ref>PMID:11283608</ref> <ref>PMID:11447600</ref> <ref>PMID:12419188</ref> <ref>PMID:12207708</ref> <ref>PMID:12810699</ref> <ref>PMID:12928491</ref> <ref>PMID:15514049</ref> <ref>PMID:15596542</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a5/3a58_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3a58 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The exocyst complex is a hetero-octameric protein complex that functions during cell polarization by tethering the secretory vesicle to the target membrane. The yeast exocyst subunit Sec3 binds to phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P(2)) and the small GTPases Rho1 and Cdc42 via its N-terminal domain (Sec3-N), and these interactions target Sec3 to the plasma membrane. Here we report the crystal structure of the Sec3-N in complex with Rho1 at 2.6-A resolution. Sec3-N adopts a pleckstrin homology (PH) fold, despite having no detectable sequence homology with other PH domains of known structure. Clusters of conserved basic residues constitute a positively charged cleft, which was identified as a binding site for PtdIns(4,5)P(2). Residues Phe77, Ile115 and Leu131 of Sec3 bind to an extended hydrophobic surface formed around switch regions I and II of Rho1. To our knowledge, these are the first structural insights into how an exocyst subunit might interact with both protein and phospholipid factors on the target membrane.
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===Crystal structure of Sec3p - Rho1p complex from Saccharomyces cerevisiae===
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Structural basis for the Rho- and phosphoinositide-dependent localization of the exocyst subunit Sec3.,Yamashita M, Kurokawa K, Sato Y, Yamagata A, Mimura H, Yoshikawa A, Sato K, Nakano A, Fukai S Nat Struct Mol Biol. 2010 Feb;17(2):180-6. Epub 2010 Jan 10. PMID:20062059<ref>PMID:20062059</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3a58" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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3A58 is a 6 chains structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A58 OCA].
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*[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]]
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[[Category: Saccharomyces cerevisiae]]
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== References ==
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[[Category: Fukai, S.]]
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<references/>
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[[Category: Mimura, H.]]
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__TOC__
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[[Category: Sato, Y.]]
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</StructureSection>
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[[Category: Yamagata, A.]]
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[[Category: Atcc 18824]]
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[[Category: Yamashita, M.]]
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[[Category: Large Structures]]
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[[Category: Yoshikawa, A.]]
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[[Category: Fukai, S]]
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[[Category: Acetylation]]
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[[Category: Mimura, H]]
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[[Category: Sato, Y]]
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[[Category: Yamagata, A]]
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[[Category: Yamashita, M]]
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[[Category: Yoshikawa, A]]
[[Category: Cell membrane]]
[[Category: Cell membrane]]
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[[Category: Coiled coil]]
 
[[Category: Endosome]]
[[Category: Endosome]]
[[Category: Exocytosis]]
[[Category: Exocytosis]]
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[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Protein transport]]
[[Category: Protein transport]]
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[[Category: Protein transport/exocytosis complex]]
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[[Category: Protein transport-exocytosis complex]]
[[Category: Transport]]
[[Category: Transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 13 14:20:14 2010''
 

Current revision

Crystal structure of Sec3p - Rho1p complex from Saccharomyces cerevisiae

PDB ID 3a58

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