1z6b

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(New page: 200px<br /><applet load="1z6b" size="450" color="white" frame="true" align="right" spinBox="true" caption="1z6b, resolution 2.09&Aring;" /> '''Crystal structure of...)
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[[Image:1z6b.gif|left|200px]]<br /><applet load="1z6b" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1z6b, resolution 2.09&Aring;" />
 
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'''Crystal structure of Plasmodium falciparum FabZ at 2.1 A'''<br />
 
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==Overview==
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==Crystal structure of Plasmodium falciparum FabZ at 2.1 A==
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The unique beta-hydroxyacyl-ACP dehydratase in Plasmodium falciparum, PfFabZ, is involved in fatty acid biosynthesis and catalyzes the, dehydration of beta-hydroxy fatty acids linked to acyl carrier protein., The structure was solved by single anomalous dispersion (SAD) phasing, using a quick-soaking experiment with potassium iodide and refined to a, resolution of 2.1 A. The crystal structure represents the first structure, of a Plasmodium beta-hydroxyacyl-ACP dehydratase with broad substrate, specificity. The asymmetric unit contains a hexamer that appears as a, trimer of dimers. Each dimer shows the known "hot dog" fold that has been, observed in only a few other protein structures. Each of the two, independent active sites in the dimer is formed by equal contributions, from both subunits. The active site is mainly hydrophobic and looks like, an L-shaped tunnel. The catalytically important amino acids His 133 and, Glu 147' (from the other subunit), together with His98', form the only, hydrophilic site in this tunnel. The inner end of the active site tunnel, is closed by the phenyl ring of Phe 169, which is located in a flexible, partly visible loop. In order to explain the acceptance of substrates, longer than ~C-7, the phenyl ring must move away to open the tunnel. The, present structure supports an enzymatic mechanism consisting of an, elimination reaction catalyzed by His 133 and Glu147'., 3-decynoyl-N-acetylcysteamine, an inhibitor known to interact with the E., coli dehydratase/isomerase, turned out to interact covalently with PfFabZ., A first model of PfFabZ with this potent inhibitor is presented.
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<StructureSection load='1z6b' size='340' side='right'caption='[[1z6b]], [[Resolution|resolution]] 2.09&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1z6b]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z6B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z6B FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.09&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z6b OCA], [https://pdbe.org/1z6b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z6b RCSB], [https://www.ebi.ac.uk/pdbsum/1z6b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z6b ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q965D7_PLAFA Q965D7_PLAFA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z6/1z6b_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1z6b ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1Z6B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum] with CL, CAC and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Z6B OCA].
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*[[Beta-hydroxyacyl-acyl carrier protein dehydratase 3D structures|Beta-hydroxyacyl-acyl carrier protein dehydratase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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The crystal structure of PfFabZ, the unique beta-hydroxyacyl-ACP dehydratase involved in fatty acid biosynthesis of Plasmodium falciparum., Kostrewa D, Winkler FK, Folkers G, Scapozza L, Perozzo R, Protein Sci. 2005 Jun;14(6):1570-80. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15930004 15930004]
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[[Category: Large Structures]]
[[Category: Plasmodium falciparum]]
[[Category: Plasmodium falciparum]]
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[[Category: Single protein]]
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[[Category: Folkers G]]
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[[Category: Folkers, G.]]
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[[Category: Kostrewa D]]
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[[Category: Kostrewa, D.]]
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[[Category: Perozzo R]]
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[[Category: Perozzo, R.]]
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[[Category: Scapozza L]]
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[[Category: Scapozza, L.]]
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[[Category: Winkler FK]]
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[[Category: Winkler, F.K.]]
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[[Category: CAC]]
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[[Category: CL]]
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[[Category: SO4]]
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[[Category: beta-hydroxyacyl-acp dehydratase]]
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[[Category: fatty acid biosynthesis]]
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[[Category: malaria]]
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[[Category: plasmodium falciparum]]
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[[Category: sad phasing]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 04:45:22 2007''
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Current revision

Crystal structure of Plasmodium falciparum FabZ at 2.1 A

PDB ID 1z6b

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