2v1p

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{{Seed}}
 
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[[Image:2v1p.png|left|200px]]
 
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==Crystal Structure of the apo form of Y74F mutant E. coli tryptophanase==
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The line below this paragraph, containing "STRUCTURE_2v1p", creates the "Structure Box" on the page.
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<StructureSection load='2v1p' size='340' side='right'caption='[[2v1p]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2v1p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V1P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V1P FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CME:S,S-(2-HYDROXYETHYL)THIOCYSTEINE'>CME</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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{{STRUCTURE_2v1p| PDB=2v1p | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v1p OCA], [https://pdbe.org/2v1p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v1p RCSB], [https://www.ebi.ac.uk/pdbsum/2v1p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v1p ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TNAA_ECOLI TNAA_ECOLI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v1/2v1p_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2v1p ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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BACKGROUND: Oligomeric enzymes can undergo a reversible loss of activity at low temperatures. One such enzyme is tryptophanase (Trpase) from Escherichia coli. Trpase is a pyridoxal phosphate (PLP)-dependent tetrameric enzyme with a Mw of 210 kD. PLP is covalently bound through an enamine bond to Lys270 at the active site. The incubation of holo E. coli Trpases at 2 degrees C for 20 h results in breaking this enamine bond and PLP release, as well as a reversible loss of activity and dissociation into dimers. This sequence of events is termed cold lability and its understanding bears relevance to protein stability and shelf life. RESULTS: We studied the reversible cold lability of E. coli Trpase and its Y74F, C298S and W330F mutants. In contrast to the holo E. coli Trpase all apo forms of Trpase dissociated into dimers already at 25 degrees C and even further upon cooling to 2 degrees C. The crystal structures of the two mutants, Y74F and C298S in their apo form were determined at 1.9A resolution. These apo mutants were found in an open conformation compared to the closed conformation found for P. vulgaris in its holo form. This conformational change is further supported by a high pressure study. CONCLUSION: We suggest that cold lability of E. coli Trpases is primarily affected by PLP release. The enhanced loss of activity of the three mutants is presumably due to the reduced size of the side chain of the amino acids. This prevents the tight assembly of the active tetramer, making it more susceptible to the cold driven changes in hydrophobic interactions which facilitate PLP release. The hydrophobic interactions along the non catalytic interface overshadow the effect of point mutations and may account for the differences in the dissociation of E. coli Trpase to dimers and P. vulgaris Trpase to monomers.
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===CRYSTAL STRUCTURE OF THE APO FORM OF Y74F MUTANT E. COLI TRYPTOPHANASE===
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Conformational changes and loose packing promote E. coli Tryptophanase cold lability.,Kogan A, Gdalevsky GY, Cohen-Luria R, Goldgur Y, Phillips RS, Parola AH, Almog O BMC Struct Biol. 2009 Oct 8;9:65. PMID:19814824<ref>PMID:19814824</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19814824}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2v1p" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19814824 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19814824}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2V1P is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V1P OCA].
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==Reference==
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<ref group="xtra">PMID:19814824</ref><references group="xtra"/>
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Tryptophanase]]
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[[Category: Large Structures]]
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[[Category: Almog, O.]]
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[[Category: Almog O]]
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[[Category: Cohen-Luria, R.]]
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[[Category: Cohen-Luria R]]
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[[Category: Gdalevsky, G Y.]]
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[[Category: Gdalevsky GY]]
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[[Category: Goldgur, Y.]]
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[[Category: Goldgur Y]]
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[[Category: Kogan, A.]]
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[[Category: Kogan A]]
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[[Category: Parola, A H.]]
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[[Category: Parola AH]]
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[[Category: Lyase]]
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[[Category: Pyridoxal phosphate]]
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[[Category: Tryptophan catabolism]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jan 18 09:35:04 2010''
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Current revision

Crystal Structure of the apo form of Y74F mutant E. coli tryptophanase

PDB ID 2v1p

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