2j74

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{{Seed}}
 
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[[Image:2j74.png|left|200px]]
 
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==Structure of Beta-1,4-Galactanase==
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The line below this paragraph, containing "STRUCTURE_2j74", creates the "Structure Box" on the page.
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<StructureSection load='2j74' size='340' side='right'caption='[[2j74]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2j74]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J74 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J74 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=PRD_900113:4beta-beta-galactobiose'>PRD_900113</scene>, <scene name='pdbligand=PRD_900114:4beta-beta-galactotriose'>PRD_900114</scene></td></tr>
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{{STRUCTURE_2j74| PDB=2j74 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j74 OCA], [https://pdbe.org/2j74 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j74 RCSB], [https://www.ebi.ac.uk/pdbsum/2j74 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j74 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GANB_BACLD GANB_BACLD] Involved in galactan degradation (PubMed:15312766). Degrades arabinose-free galactan to galactooligosaccharides, producing galactotetraose as the main product along with galactotriose, galactobiose, and galactose (PubMed:15312766). May hydrolyze the beta-1,4-galactan linkages of the galactan portion of arabinogalactan type I, a pectic plant polysaccharide from which most of the arabinose has been removed (By similarity).[UniProtKB:O07013]<ref>PMID:15312766</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j7/2j74_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2j74 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Microbial beta-1,4-galactanases are glycoside hydrolases belonging to family 53, which degrade galactan and arabinogalactan side chains in the hairy regions of pectin, a major plant cell wall component. They belong to the larger clan GH-A of glycoside hydrolases, which cover many different poly- and oligosaccharidase specificities. Crystallographic complexes of Bacillus licheniformis beta-1,4-galactanase and its inactive nucleophile mutant have been obtained with methyl-beta(1--&gt;4)-galactotetraoside, providing, for the first time, information on substrate binding to the aglycone side of the beta-1,4-galactanase substrate binding groove. Using the experimentally determined subsites as a starting point, a beta(1--&gt;4)-galactononaose was built into the structure and subjected to molecular dynamics simulations giving further insight into the residues involved in the binding of the polysaccharide from subsite -4 to +5. In particular, this analysis newly identified a conserved beta-turn, which contributes to subsites -2 to +3. This beta-turn is unique to family 53 beta-1,4-galactanases among all clan GH-A families that have been structurally characterized and thus might be a structural signature for endo-beta-1,4-galactanase specificity.
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===STRUCTURE OF BETA-1,4-GALACTANASE===
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Investigating the binding of beta-1,4-galactan to Bacillus licheniformis beta-1,4-galactanase by crystallography and computational modeling.,Le Nours J, De Maria L, Welner D, Jorgensen CT, Christensen LL, Borchert TV, Larsen S, Lo Leggio L Proteins. 2009 Jun;75(4):977-89. PMID:19089956<ref>PMID:19089956</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2j74" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19089956}}, adds the Publication Abstract to the page
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*[[Beta-1%2C4-galactanase|Beta-1%2C4-galactanase]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19089956 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19089956}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2J74 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J74 OCA].
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==Reference==
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<ref group="xtra">PMID:19089956</ref><references group="xtra"/>
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[[Category: Arabinogalactan endo-1,4-beta-galactosidase]]
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[[Category: Bacillus licheniformis]]
[[Category: Bacillus licheniformis]]
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[[Category: Christensen, L L.H.]]
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[[Category: Large Structures]]
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[[Category: Jorgensen, C T.]]
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[[Category: Christensen LLH]]
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[[Category: Larsen, S.]]
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[[Category: De Maria L]]
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[[Category: Leggio, L Lo.]]
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[[Category: Jorgensen CT]]
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[[Category: Maria, L De.]]
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[[Category: Larsen S]]
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[[Category: Nours, J Le.]]
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[[Category: Le Nours J]]
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[[Category: Welner, D.]]
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[[Category: Lo Leggio L]]
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[[Category: 4-galactanase]]
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[[Category: Welner D]]
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[[Category: Beta-1]]
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[[Category: Galactan]]
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[[Category: Gh-a]]
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[[Category: Glycoside hydrolase family 53]]
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[[Category: Hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jan 18 10:33:20 2010''
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Current revision

Structure of Beta-1,4-Galactanase

PDB ID 2j74

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