1z7r

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1z7r" size="450" color="white" frame="true" align="right" spinBox="true" caption="1z7r" /> '''Solution Structure of reduced glutaredoxin C...)
Current revision (06:37, 1 May 2024) (edit) (undo)
 
(14 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1z7r.gif|left|200px]]<br /><applet load="1z7r" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1z7r" />
 
-
'''Solution Structure of reduced glutaredoxin C1 from Populus tremula x tremuloides'''<br />
 
-
==Overview==
+
==Solution Structure of reduced glutaredoxin C1 from Populus tremula x tremuloides==
-
Glutaredoxins are glutathione-dependent enzymes that function to reduce, disulfide bonds in vivo. Interestingly, a recent discovery indicates that, some glutaredoxins can also exist in another form, an iron-sulfur protein, [Lillig, C. H., et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102, 8168-8173]. This provides a direct connection between glutaredoxins and, iron-sulfur proteins, suggesting a possible new regulatory role of, iron-sulfur clusters along with the new functional switch of, glutaredoxins. Biochemical studies have indicated that poplar glutaredoxin, C1 (Grx-C1) is also such a biform protein. The apo form (monomer) of, Grx-C1 is a regular glutaredoxin, and the holo form (dimer) is an, iron-sulfur protein with a bridging [2Fe-2S] cluster. Here, we report the, structural characterizations of poplar Grx-C1 in both the apo and holo, forms by NMR spectroscopy. The solution structure of the reduced apo, Grx-C1, which is the first plant Grx structure, shows a typical Grx fold., When poplar Grx-C1 forms a dimer with an iron-sulfur cluster, each subunit, of the holo form still retains the overall fold of the apo form. The, bridging iron-sulfur cluster in holo Grx-C1 is coordinated near the active, site. In addition to the iron-sulfur cluster linker, helix alpha3 of each, subunit is probably involved in the direct contact between the two, subunits. Moreover, two glutathione molecules are identified in the, vicinity of the iron-sulfur cluster and very likely participate in cluster, coordination. Taken together, we propose that the bridging [2Fe-2S], cluster is coordinated by the first cysteine at the glutaredoxin active, site from each subunit of holo Grx-C1, along with two cysteines from two, glutathione molecules. Our studies reveal that holo Grx-C1 has a novel, structural and iron-sulfur cluster coordination pattern for an iron-sulfur, protein.
+
<StructureSection load='1z7r' size='340' side='right'caption='[[1z7r]]' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1z7r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Populus_tremula_x_Populus_tremuloides Populus tremula x Populus tremuloides]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z7R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z7R FirstGlance]. <br>
-
1Z7R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Populus_tremula_x_populus_tremuloides Populus tremula x populus tremuloides]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Z7R OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-
 
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z7r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z7r OCA], [https://pdbe.org/1z7r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z7r RCSB], [https://www.ebi.ac.uk/pdbsum/1z7r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z7r ProSAT]</span></td></tr>
-
==Reference==
+
</table>
-
Structural insight into poplar glutaredoxin C1 with a bridging iron-sulfur cluster at the active site., Feng Y, Zhong N, Rouhier N, Hase T, Kusunoki M, Jacquot JP, Jin C, Xia B, Biochemistry. 2006 Jul 4;45(26):7998-8008. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16800625 16800625]
+
== Function ==
-
[[Category: Populus tremula x populus tremuloides]]
+
[https://www.uniprot.org/uniprot/Q5PSJ1_POPPZ Q5PSJ1_POPPZ]
-
[[Category: Single protein]]
+
== Evolutionary Conservation ==
-
[[Category: Feng, Y.]]
+
[[Image:Consurf_key_small.gif|200px|right]]
-
[[Category: Jacquot, J.P.]]
+
Check<jmol>
-
[[Category: Rouhier, N.]]
+
<jmolCheckbox>
-
[[Category: Xia, B.]]
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z7/1z7r_consurf.spt"</scriptWhenChecked>
-
[[Category: Zhong, N.]]
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
[[Category: electron transport]]
+
<text>to colour the structure by Evolutionary Conservation</text>
-
 
+
</jmolCheckbox>
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 04:46:31 2007''
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1z7r ConSurf].
 +
<div style="clear:both"></div>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
 +
[[Category: Populus tremula x Populus tremuloides]]
 +
[[Category: Feng Y]]
 +
[[Category: Jacquot JP]]
 +
[[Category: Rouhier N]]
 +
[[Category: Xia B]]
 +
[[Category: Zhong N]]

Current revision

Solution Structure of reduced glutaredoxin C1 from Populus tremula x tremuloides

PDB ID 1z7r

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1z7r"
Personal tools