1zat

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(New page: 200px<br /><applet load="1zat" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zat, resolution 2.40&Aring;" /> '''Crystal Structure of...)
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[[Image:1zat.gif|left|200px]]<br /><applet load="1zat" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1zat, resolution 2.40&Aring;" />
 
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'''Crystal Structure of an Enterococcus faecium peptidoglycan binding protein at 2.4 A resolution'''<br />
 
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==Overview==
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==Crystal Structure of an Enterococcus faecium peptidoglycan binding protein at 2.4 A resolution==
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During the final stages of cell-wall synthesis in bacteria, penicillin-binding proteins (PBPs) catalyse the cross-linking of peptide, chains from adjacent glycan strands of nascent peptidoglycan. We have, recently shown that this step can be bypassed by an L,D-transpeptidase, which confers high-level beta-lactam-resistance in Enterococcus faecium., The resistance bypass leads to replacement of D-Ala4--&gt;D-Asx-L-Lys3, cross-links generated by the PBPs by L-Lys3--&gt;D-Asx-L-Lys3 cross-links, generated by the L,D-transpeptidase. As the first structure of a member of, this new transpeptidase family, we have determined the crystal structure, of a fragment of the L,D-transpeptidase from E.faecium (Ldt(fm217)) at, 2.4A resolution. Ldt(fm217) consists of two domains, the N-terminal, domain, a new mixed alpha-beta fold, and the ErfK_YbiS_YhnG C-terminal, domain, a representative of the mainly beta class of protein structures., Residue Cys442 of the C-terminal domain has been proposed to be the, catalytic residue implicated in the cleavage of the L-Lys-D-Ala peptide, bond. Surface analysis of Ldt(fm217) reveals that residue Cys442 is, localized in a buried pocket and is accessible by two paths on different, sides of the protein. We propose that the two paths to the catalytic, residue Cys442 are the binding sites for the acceptor and donor substrates, of the L,D-transpeptidase.
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<StructureSection load='1zat' size='340' side='right'caption='[[1zat]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1zat]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecium Enterococcus faecium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZAT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZAT FirstGlance]. <br>
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1ZAT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecium Enterococcus faecium] with ZN and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZAT OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zat OCA], [https://pdbe.org/1zat PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zat RCSB], [https://www.ebi.ac.uk/pdbsum/1zat PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zat ProSAT]</span></td></tr>
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Crystal structure of a novel beta-lactam-insensitive peptidoglycan transpeptidase., Biarrotte-Sorin S, Hugonnet JE, Delfosse V, Mainardi JL, Gutmann L, Arthur M, Mayer C, J Mol Biol. 2006 Jun 9;359(3):533-8. Epub 2006 Mar 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16647082 16647082]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q3Y185_ENTFD Q3Y185_ENTFD]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/za/1zat_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zat ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Enterococcus faecium]]
[[Category: Enterococcus faecium]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Arthur, M.]]
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[[Category: Arthur M]]
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[[Category: Biarrotte-Sorin, S.]]
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[[Category: Biarrotte-Sorin S]]
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[[Category: Gutmann, L.]]
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[[Category: Gutmann L]]
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[[Category: Hugonnet, J.E.]]
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[[Category: Hugonnet J-E]]
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[[Category: Mainardi, J.L.]]
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[[Category: Mainardi J-L]]
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[[Category: Mayer, C.]]
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[[Category: Mayer C]]
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[[Category: Rice, L.]]
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[[Category: Rice L]]
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[[Category: SO4]]
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[[Category: ZN]]
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[[Category: antibiotic resistance]]
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[[Category: beta-lactam insensitive transpeptidase]]
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[[Category: d-transpeptidation]]
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[[Category: l]]
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[[Category: peptidoglycan]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 04:48:14 2007''
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Current revision

Crystal Structure of an Enterococcus faecium peptidoglycan binding protein at 2.4 A resolution

PDB ID 1zat

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