3keg

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{{Seed}}
 
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[[Image:3keg.png|left|200px]]
 
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==X-ray Crystallographic Structure of a Y131F mutant of Pseudomonas Aeruginosa Azoreductase in complex with Methyl RED==
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The line below this paragraph, containing "STRUCTURE_3keg", creates the "Structure Box" on the page.
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<StructureSection load='3keg' size='340' side='right'caption='[[3keg]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3keg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KEG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KEG FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MRE:2-(4-DIMETHYLAMINOPHENYL)DIAZENYLBENZOIC+ACID'>MRE</scene></td></tr>
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{{STRUCTURE_3keg| PDB=3keg | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3keg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3keg OCA], [https://pdbe.org/3keg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3keg RCSB], [https://www.ebi.ac.uk/pdbsum/3keg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3keg ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AZOR1_PSEAE AZOR1_PSEAE] Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ke/3keg_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3keg ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Azoreductase 1 from Pseudomonas aeruginosa strain PAO1 (paAzoR1) catalyses the activation of the prodrug balsalazide and reduces the azo dye methyl red using reduced nicotinamide adenine dinucleotide cofactor as an electron donor. To investigate the mechanism of the enzyme, a Y131F mutation was introduced and the enzymic properties of the mutant were compared with those of the wild-type enzyme. The crystallographic structure of the mutant with methyl red bound was solved at 2.1 A resolution and compared with the wild-type structure. Tyr131 is important in the architecture of the active site but is not essential for enzymic activity.
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===X-ray Crystallographic Structure of a Y131F mutant of Pseudomonas Aeruginosa Azoreductase in complex with Methyl RED===
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Role of tyrosine 131 in the active site of paAzoR1, an azoreductase with specificity for the inflammatory bowel disease prodrug balsalazide.,Wang CJ, Laurieri N, Abuhammad A, Lowe E, Westwood I, Ryan A, Sim E Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Jan 1;66(Pt 1):2-7., Epub 2009 Dec 25. PMID:20057057<ref>PMID:20057057</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20057057}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3keg" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20057057 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20057057}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3KEG is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KEG OCA].
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[[Category: Pseudomonas aeruginosa PAO1]]
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[[Category: Abuhammad A]]
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==Reference==
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[[Category: Laurieri N]]
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<ref group="xtra">PMID:20057057</ref><references group="xtra"/>
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[[Category: Lowe E]]
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[[Category: Pseudomonas aeruginosa]]
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[[Category: Ryan A]]
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[[Category: Abuhammad, A.]]
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[[Category: Sim E]]
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[[Category: Laurieri, N.]]
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[[Category: Wang C-J]]
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[[Category: Lowe, E.]]
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[[Category: Westwood I]]
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[[Category: Ryan, A.]]
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[[Category: Sim, E.]]
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[[Category: Wang, C J.]]
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[[Category: Westwood, I.]]
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[[Category: Flavoprotein]]
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[[Category: Fmn]]
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[[Category: Methyl red]]
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[[Category: Nad]]
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[[Category: Oxidoreductase]]
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[[Category: Y131f azoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 20 08:44:47 2010''
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Current revision

X-ray Crystallographic Structure of a Y131F mutant of Pseudomonas Aeruginosa Azoreductase in complex with Methyl RED

PDB ID 3keg

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