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1zjc

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(New page: 200px<br /><applet load="1zjc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zjc, resolution 1.80&Aring;" /> '''Aminopeptidase S fro...)
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[[Image:1zjc.gif|left|200px]]<br /><applet load="1zjc" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1zjc, resolution 1.80&Aring;" />
 
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'''Aminopeptidase S from S. aureus'''<br />
 
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==Overview==
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==Aminopeptidase S from S. aureus==
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Staphylococcus aureus aminopeptidase S (AmpS) has been named for its, predicted, but experimentally untested, aminopeptidase activity. The, enzyme is homologous to biochemically characterized aminopeptidases that, contain two cobalt or zinc ions in their active centers, but it is, unrelated to all structurally characterized metallopeptidases. Here, we, demonstrate AmpS aminopeptidase activity experimentally, and we present, the 1.8-A crystal structure of the enzyme. Two metal ions with full, occupancy and a third metal ion with low occupancy are present in the, active site. A water molecule and Glu-319 serve as bridging ligands to the, two metals with full occupancy. One of these metal ions is additionally, coordinated by Glu-253 and His-348 and the other by His-381 and Asp-383., In addition, the metals are involved in weak metal-donor interactions to a, water molecule and to Tyr-355. In the crystal, AmpS forms a dimer with a, large internal cavity. The active sites are located at opposite ends of, this internal cavity and are essentially inaccessible from the outside, suggesting that an inactive conformation was crystallized. Because gel, filtration and analytical ultracentrifugation data also suggest dimer, formation, the problem of substrate access to the active site cavity, remains unresolved.
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<StructureSection load='1zjc' size='340' side='right'caption='[[1zjc]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1zjc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_MW2 Staphylococcus aureus subsp. aureus MW2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZJC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZJC FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zjc OCA], [https://pdbe.org/1zjc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zjc RCSB], [https://www.ebi.ac.uk/pdbsum/1zjc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zjc ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A0A0H3K3S3_STAAW A0A0H3K3S3_STAAW]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zj/1zjc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zjc ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1ZJC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with CO as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZJC OCA].
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*[[Aminopeptidase 3D structures|Aminopeptidase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Staphylococcus aureus aminopeptidase S is a founding member of a new peptidase clan., Odintsov SG, Sabala I, Bourenkov G, Rybin V, Bochtler M, J Biol Chem. 2005 Jul 29;280(30):27792-9. Epub 2005 Jun 2. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15932875 15932875]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Staphylococcus aureus subsp. aureus MW2]]
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[[Category: Staphylococcus aureus]]
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[[Category: Bochtler M]]
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[[Category: Bochtler, M.]]
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[[Category: Bourenkov G]]
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[[Category: Bourenkov, G.]]
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[[Category: Odintsov SG]]
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[[Category: Odintsov, S.G.]]
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[[Category: Rybin V]]
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[[Category: Rybin, V.]]
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[[Category: Sabala I]]
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[[Category: Sabala, I.]]
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[[Category: CO]]
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[[Category: aminopeptidase]]
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[[Category: metallopeptidase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 04:54:18 2007''
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Current revision

Aminopeptidase S from S. aureus

PDB ID 1zjc

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