1r8j

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (06:02, 23 August 2023) (edit) (undo)
 
(8 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:1r8j.png|left|200px]]
 
-
<!--
+
==Crystal Structure of Circadian Clock Protein KaiA from Synechococcus elongatus==
-
The line below this paragraph, containing "STRUCTURE_1r8j", creates the "Structure Box" on the page.
+
<StructureSection load='1r8j' size='340' side='right'caption='[[1r8j]], [[Resolution|resolution]] 2.03&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1r8j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechococcus_elongatus_PCC_7942_=_FACHB-805 Synechococcus elongatus PCC 7942 = FACHB-805]. The January 2008 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Circadian Clock Proteins'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2008_1 10.2210/rcsb_pdb/mom_2008_1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R8J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R8J FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.03&#8491;</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r8j OCA], [https://pdbe.org/1r8j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r8j RCSB], [https://www.ebi.ac.uk/pdbsum/1r8j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r8j ProSAT]</span></td></tr>
-
{{STRUCTURE_1r8j| PDB=1r8j | SCENE= }}
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/KAIA_SYNE7 KAIA_SYNE7] Component of the KaiABC clock protein complex, which constitutes the main circadian regulator in cyanobacteria. The KaiABC complex may act as a promoter-nonspecific transcription regulator that represses transcription, possibly by acting on the state of chromosome compaction. In the complex, it enhances the phosphorylation status of KaiC. In contrast, the presence of KaiB in the complex decreases the phosphorylation status of KaiC, suggesting that KaiB acts by antagonizing the interaction between KaiA and KaiC. A KaiA dimer is sufficient to enhance KaiC hexamer phosphorylation.<ref>PMID:12391300</ref> <ref>PMID:12727878</ref> <ref>PMID:12727879</ref> <ref>PMID:9727980</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r8/1r8j_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r8j ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The circadian clock found in Synechococcus elongatus, the most ancient circadian clock, is regulated by the interaction of three proteins, KaiA, KaiB, and KaiC. While the precise function of these proteins remains unclear, KaiA has been shown to be a positive regulator of the expression of KaiB and KaiC. The 2.0-A structure of KaiA of S. elongatus reported here shows that the protein is composed of two independently folded domains connected by a linker. The NH(2)-terminal pseudo-receiver domain has a similar fold with that of bacterial response regulators, whereas the COOH-terminal four-helix bundle domain is novel and forms the interface of the 2-fold-related homodimer. The COOH-terminal four-helix bundle domain has been shown to contain the KaiC binding site. The structure suggests that the KaiB binding site is covered in the dimer interface of the KaiA "closed" conformation, observed in the crystal structure, which suggests an allosteric regulation mechanism.
-
===Crystal Structure of Circadian Clock Protein KaiA from Synechococcus elongatus===
+
Crystal structure of circadian clock protein KaiA from Synechococcus elongatus.,Ye S, Vakonakis I, Ioerger TR, LiWang AC, Sacchettini JC J Biol Chem. 2004 May 7;279(19):20511-8. Epub 2004 Mar 8. PMID:15007067<ref>PMID:15007067</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 1r8j" style="background-color:#fffaf0;"></div>
-
<!--
+
==See Also==
-
The line below this paragraph, {{ABSTRACT_PUBMED_15007067}}, adds the Publication Abstract to the page
+
*[[Circadian clock protein|Circadian clock protein]]
-
(as it appears on PubMed at http://www.pubmed.gov), where 15007067 is the PubMed ID number.
+
*[[Circadian clock protein 3D structures|Circadian clock protein 3D structures]]
-
-->
+
== References ==
-
{{ABSTRACT_PUBMED_15007067}}
+
<references/>
-
 
+
__TOC__
-
==About this Structure==
+
</StructureSection>
-
1R8J is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Synechococcus_elongatus_pcc_7942 Synechococcus elongatus pcc 7942]. The January 2008 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Circadian Clock Proteins'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2008_1 10.2210/rcsb_pdb/mom_2008_1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R8J OCA].
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:15007067</ref><references group="xtra"/>
+
[[Category: Circadian Clock Proteins]]
[[Category: Circadian Clock Proteins]]
 +
[[Category: Large Structures]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: RCSB PDB Molecule of the Month]]
-
[[Category: Synechococcus elongatus pcc 7942]]
+
[[Category: Synechococcus elongatus PCC 7942 = FACHB-805]]
-
[[Category: LiWang, A C.]]
+
[[Category: LiWang AC]]
-
[[Category: Sacchettini, J C.]]
+
[[Category: Sacchettini JC]]
-
[[Category: Vakonakis, I.]]
+
[[Category: Vakonakis I]]
-
[[Category: Ye, S.]]
+
[[Category: Ye S]]
-
[[Category: Circadian clock protein]]
+
-
[[Category: Kaia]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jan 21 08:55:11 2010''
+

Current revision

Crystal Structure of Circadian Clock Protein KaiA from Synechococcus elongatus

PDB ID 1r8j

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1r8j"
Personal tools