1ezg

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{{Seed}}
 
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[[Image:1ezg.png|left|200px]]
 
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==CRYSTAL STRUCTURE OF ANTIFREEZE PROTEIN FROM THE BEETLE, TENEBRIO MOLITOR==
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The line below this paragraph, containing "STRUCTURE_1ezg", creates the "Structure Box" on the page.
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<StructureSection load='1ezg' size='340' side='right'caption='[[1ezg]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1ezg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Tenebrio_molitor Tenebrio molitor]. The December 2009 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Antifreeze Proteins'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2009_12 10.2210/rcsb_pdb/mom_2009_12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EZG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EZG FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ezg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ezg OCA], [https://pdbe.org/1ezg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ezg RCSB], [https://www.ebi.ac.uk/pdbsum/1ezg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ezg ProSAT]</span></td></tr>
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{{STRUCTURE_1ezg| PDB=1ezg | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ANPY1_TENMO ANPY1_TENMO] Contributes to protect body fluid from freezing at subzero temperatures. Lowers the freezing point of the hemolymph by about 2.5 degrees at a concentration of 1 mg/ml. Binds to nascent ice crystals and prevents further growth.<ref>PMID:10471292</ref> <ref>PMID:10833402</ref> <ref>PMID:9285581</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ez/1ezg_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ezg ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Insect antifreeze proteins (AFP) are much more effective than fish AFPs at depressing solution freezing points by ice-growth inhibition. AFP from the beetle Tenebrio molitor is a small protein (8.4 kDa) composed of tandem 12-residue repeats (TCTxSxxCxxAx). Here we report its 1.4-A resolution crystal structure, showing that this repetitive sequence translates into an exceptionally regular beta-helix. Not only are the 12-amino-acid loops almost identical in the backbone, but also the conserved side chains are positioned in essentially identical orientations, making this AFP perhaps the most regular protein structure yet observed. The protein has almost no hydrophobic core but is stabilized by numerous disulphide and hydrogen bonds. On the conserved side of the protein, threonine-cysteine-threonine motifs are arrayed to form a flat beta-sheet, the putative ice-binding surface. The threonine side chains have exactly the same rotameric conformation and the spacing between OH groups is a near-perfect match to the ice lattice. Together with tightly bound co-planar external water, three ranks of oxygen atoms form a two-dimensional array, mimicking an ice section.
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===CRYSTAL STRUCTURE OF ANTIFREEZE PROTEIN FROM THE BEETLE, TENEBRIO MOLITOR===
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Mimicry of ice structure by surface hydroxyls and water of a beta-helix antifreeze protein.,Liou YC, Tocilj A, Davies PL, Jia Z Nature. 2000 Jul 20;406(6793):322-4. PMID:10917536<ref>PMID:10917536</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1ezg" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_10917536}}, adds the Publication Abstract to the page
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*[[Antifreeze protein 3D structures|Antifreeze protein 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 10917536 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_10917536}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1EZG is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Tenebrio_molitor Tenebrio molitor]. The December 2009 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Antifreeze Proteins'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2009_12 10.2210/rcsb_pdb/mom_2009_12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EZG OCA].
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==Reference==
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<ref group="xtra">PMID:10917536</ref><references group="xtra"/>
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[[Category: Antifreeze Proteins]]
[[Category: Antifreeze Proteins]]
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[[Category: Large Structures]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Tenebrio molitor]]
[[Category: Tenebrio molitor]]
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[[Category: Davies, P L.]]
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[[Category: Davies PL]]
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[[Category: Jia, Z.]]
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[[Category: Jia Z]]
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[[Category: Liou, Y C.]]
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[[Category: Liou Y-C]]
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[[Category: Tocilj, A.]]
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[[Category: Tocilj A]]
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[[Category: Insect antifreeze protein]]
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[[Category: Iodination]]
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[[Category: Right-handed beta-helix]]
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[[Category: Tenebrio molitor]]
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[[Category: Thermal hysteresis]]
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[[Category: Tmafp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jan 21 10:07:20 2010''
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Current revision

CRYSTAL STRUCTURE OF ANTIFREEZE PROTEIN FROM THE BEETLE, TENEBRIO MOLITOR

PDB ID 1ezg

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