2kr4

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{{Seed}}
 
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[[Image:2kr4.jpg|left|200px]]
 
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==U-box domain of the E3 Ubiquitin Ligase E4B==
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The line below this paragraph, containing "STRUCTURE_2kr4", creates the "Structure Box" on the page.
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<StructureSection load='2kr4' size='340' side='right'caption='[[2kr4]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2kr4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KR4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KR4 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kr4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kr4 OCA], [https://pdbe.org/2kr4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kr4 RCSB], [https://www.ebi.ac.uk/pdbsum/2kr4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kr4 ProSAT]</span></td></tr>
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{{STRUCTURE_2kr4| PDB=2kr4 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/UBE4B_MOUSE UBE4B_MOUSE] Binds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3 (By similarity).<ref>PMID:11770485</ref> [UniProtKB:O95155]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kr/2kr4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kr4 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Substantial evidence has accumulated indicating a significant role for oligomerization in the function of E3 ubiquitin ligases. Among the many characterized E3 ligases, the yeast U-box protein Ufd2 and its mammalian homologue E4B appear to be unique in functioning as monomers. An E4B U-box domain construct (E4BU) has been subcloned, overexpressed in Escherichia coli, and purified, which enabled determination of a high-resolution NMR solution structure and detailed biophysical analysis. E4BU is a stable monomeric protein that folds into the same structure observed for other structurally characterized U-box domain homodimers. Multiple sequence alignment combined with comparative structural analysis reveals substitutions in the sequence that inhibit dimerization. The interaction between E4BU and the E2 conjugating enzyme UbcH5c has been mapped using NMR, and these data have been used to generate a structural model for the complex. The E2 binding site is found to be similar to that observed for dimeric U-box and RING domain E3 ligases. Despite the inability to dimerize, E4BU was found to be active in a standard autoubiquitination assay. The structure of E4BU and its ability to function as a monomer are discussed in light of the ubiquitous observation of U-box and RING domain oligomerization.
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===U-box domain of the E3 Ubiquitin Ligase E4B===
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Structural and functional characterization of the monomeric U-box domain from E4B.,Nordquist KA, Dimitrova YN, Brzovic PS, Ridenour WB, Munro KA, Soss SE, Caprioli RM, Klevit RE, Chazin WJ Biochemistry. 2010 Jan 19;49(2):347-55. PMID:20017557<ref>PMID:20017557</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20017557}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2kr4" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20017557 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20017557}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2KR4 is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KR4 OCA].
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==Reference==
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<ref group="xtra">PMID:20017557</ref><references group="xtra"/>
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[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Chazin, W.]]
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[[Category: Chazin W]]
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[[Category: Nordquist, K.]]
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[[Category: Nordquist K]]
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[[Category: Soss, S.]]
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[[Category: Soss S]]
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[[Category: E3 ligase]]
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[[Category: E4b]]
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[[Category: Ring]]
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[[Category: U-box]]
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[[Category: Ubiquitin]]
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[[Category: Ubl conjugation pathway]]
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[[Category: Ufd2]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 27 19:31:09 2010''
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Current revision

U-box domain of the E3 Ubiquitin Ligase E4B

PDB ID 2kr4

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