2zbn

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{{Seed}}
 
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[[Image:2zbn.png|left|200px]]
 
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==Crystal structure of PH1033 from Pyrococcus horikoshii OT3==
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The line below this paragraph, containing "STRUCTURE_2zbn", creates the "Structure Box" on the page.
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<StructureSection load='2zbn' size='340' side='right'caption='[[2zbn]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2zbn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii_OT3 Pyrococcus horikoshii OT3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZBN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZBN FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zbn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zbn OCA], [https://pdbe.org/2zbn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zbn RCSB], [https://www.ebi.ac.uk/pdbsum/2zbn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zbn ProSAT]</span></td></tr>
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{{STRUCTURE_2zbn| PDB=2zbn | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Y1033_PYRHO Y1033_PYRHO]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zb/2zbn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zbn ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Protein crystallization is still a major bottleneck in structural biology. As the current methodology of protein crystallization is a type of screening, it is usually difficult to crystallize important target proteins. It was thought that hetero-epitaxic growth from the surface of a mineral crystal acting as a nucleant would be an effective enhancer of protein crystallization. However, in spite of almost two decades of effort, a generally applicable hetero-epitaxic nucleant for protein crystallization has yet to be found. Here we introduce the first candidate for a universal hetero-epitaxic nucleant, microporous zeolite: a synthetic aluminosilicate crystalline polymer with regular micropores. It promotes a form-selective crystal nucleation of proteins and acts as a crystallization catalyst. The most successful zeolite nucleant was molecular sieve type 5A with a pore size of 5 A and with bound Ca2+ ions. The zeolite-mediated crystallization improved the crystal quality in five out of six proteins tested. It provided new crystal forms with better resolution in two cases, larger crystals in one case, and zeolite-dependent crystal formations in two cases. The hetero-epitaxic growth of the zeolite-mediated crystals was confirmed by a crystal-packing analysis which revealed a layer-like structure in the crystal lattice.
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===Crystal structure of PH1033 from Pyrococcus horikoshii OT3===
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Nucleant-mediated protein crystallization with the application of microporous synthetic zeolites.,Sugahara M, Asada Y, Morikawa Y, Kageyama Y, Kunishima N Acta Crystallogr D Biol Crystallogr. 2008 Jun;64(Pt 6):686-95. Epub 2008, May 14. PMID:18560157<ref>PMID:18560157</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<!--
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_18560157}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2zbn" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 18560157 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18560157}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2ZBN is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii_ot3 Pyrococcus horikoshii ot3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZBN OCA].
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[[Category: Pyrococcus horikoshii OT3]]
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[[Category: Kunishima N]]
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==Reference==
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[[Category: Sugahara M]]
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<ref group="xtra">PMID:18560157</ref><references group="xtra"/>
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[[Category: Pyrococcus horikoshii ot3]]
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[[Category: Kunishima, N.]]
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[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]
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[[Category: Sugahara, M.]]
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[[Category: National project on protein structural and functional analyse]]
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[[Category: Nppsfa]]
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[[Category: Pyrococcus horikoshii ot3]]
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[[Category: Riken structural genomics/proteomics initiative]]
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[[Category: Rsgi]]
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[[Category: Structural genomic]]
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[[Category: Unknown function]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 27 19:38:25 2010''
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Current revision

Crystal structure of PH1033 from Pyrococcus horikoshii OT3

PDB ID 2zbn

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