1bou

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[[Image:1bou.gif|left|200px]]<br />
 
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<applet load="1bou" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1bou, resolution 2.2&Aring;" />
 
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'''THREE-DIMENSIONAL STRUCTURE OF LIGAB'''<br />
 
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==Overview==
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==THREE-DIMENSIONAL STRUCTURE OF LIGAB==
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BACKGROUND: Sphingomonas paucimobilis SYK-6 utilizes an extradiol-type, catecholic dioxygenase, the LigAB enzyme (a protocatechuate, 4,5-dioxygenase), to oxidize protocatechuate (or 3,4-dihydroxybenzoic, acid, PCA). The enzyme belongs to the family of class III extradiol-type, catecholic dioxygenases catalyzing the ring-opening reaction of, protocatechuate and related compounds. The primary structure of LigAB, suggests that the enzyme has no evolutionary relationship with the family, of class II extradiol-type catecholic dioxygenases. Both the class II and, class III enzymes utilize a non-heme ferrous center for adding dioxygen to, the substrate. By elucidating the structure of LigAB, we aimed to provide, a structural basis for discussing the function of class III enzymes., RESULTS: The ... [[http://ispc.weizmann.ac.il/pmbin/getpm?10467151 (full description)]]
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<StructureSection load='1bou' size='340' side='right'caption='[[1bou]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1bou]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Sphingomonas_paucimobilis Sphingomonas paucimobilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BOU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BOU FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bou OCA], [https://pdbe.org/1bou PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bou RCSB], [https://www.ebi.ac.uk/pdbsum/1bou PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bou ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PCYA_SPHSK PCYA_SPHSK]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bo/1bou_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bou ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1BOU is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Sphingomonas_paucimobilis Sphingomonas paucimobilis]] with FE as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Protocatechuate_4,5-dioxygenase Protocatechuate 4,5-dioxygenase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.11.8 1.13.11.8]]. Structure known Active Sites: FEA and FEB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BOU OCA]].
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*[[Dioxygenase 3D structures|Dioxygenase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of an aromatic ring opening dioxygenase LigAB, a protocatechuate 4,5-dioxygenase, under aerobic conditions., Sugimoto K, Senda T, Aoshima H, Masai E, Fukuda M, Mitsui Y, Structure. 1999 Aug 15;7(8):953-65. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10467151 10467151]
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[[Category: Large Structures]]
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[[Category: Protein complex]]
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[[Category: Protocatechuate 4,5-dioxygenase]]
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[[Category: Sphingomonas paucimobilis]]
[[Category: Sphingomonas paucimobilis]]
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[[Category: Fukuda, M.]]
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[[Category: Fukuda M]]
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[[Category: Mitsui, Y.]]
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[[Category: Mitsui Y]]
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[[Category: Senda, T.]]
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[[Category: Senda T]]
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[[Category: Sugimoto, K.]]
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[[Category: Sugimoto K]]
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[[Category: FE]]
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[[Category: extradiol type dioxygenase]]
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[[Category: oxidoreductase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 10:03:36 2007''
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Current revision

THREE-DIMENSIONAL STRUCTURE OF LIGAB

PDB ID 1bou

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