3h6d

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{{Seed}}
 
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[[Image:3h6d.png|left|200px]]
 
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==Structure of the mycobacterium tuberculosis DUTPase D28N mutant==
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The line below this paragraph, containing "STRUCTURE_3h6d", creates the "Structure Box" on the page.
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<StructureSection load='3h6d' size='340' side='right'caption='[[3h6d]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3h6d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H6D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3H6D FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DUP:2-DEOXYURIDINE+5-ALPHA,BETA-IMIDO-TRIPHOSPHATE'>DUP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
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{{STRUCTURE_3h6d| PDB=3h6d | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3h6d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3h6d OCA], [https://pdbe.org/3h6d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3h6d RCSB], [https://www.ebi.ac.uk/pdbsum/3h6d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3h6d ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DUT_MYCTU DUT_MYCTU] This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.[HAMAP-Rule:MF_00116]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h6/3h6d_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3h6d ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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dUTP pyrophosphatases (dUTPases) are essential for genome integrity. Recent results allowed characterization of the role of conserved residues. Here we analyzed the Asp/Asn mutation within conserved Motif I of human and mycobacterial dUTPases, wherein the Asp residue was previously implicated in Mg(2+)-coordination. Our results on transient/steady-state kinetics, ligand binding and a 1.80 A resolution structure of the mutant mycobacterial enzyme, in comparison with wild type and C-terminally truncated structures, argue that this residue has a major role in providing intra- and intersubunit contacts, but is not essential for Mg(2+) accommodation. We conclude that in addition to the role of conserved motifs in substrate accommodation, direct subunit interaction between protein atoms of active site residues from different conserved motifs are crucial for enzyme function.
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===Structure of the mycobacterium tuberculosis DUTPase D28N mutant===
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Direct contacts between conserved motifs of different subunits provide major contribution to active site organization in human and mycobacterial dUTPases.,Takacs E, Nagy G, Leveles I, Harmat V, Lopata A, Toth J, Vertessy BG FEBS Lett. 2010 Jul 16;584(14):3047-54. Epub 2010 May 21. PMID:20493855<ref>PMID:20493855</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3h6d" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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3H6D is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H6D OCA].
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*[[DUTPase 3D structures|DUTPase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
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[[Category: DUTP diphosphatase]]
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[[Category: Harmat V]]
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[[Category: Harmat, V.]]
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[[Category: Leveles I]]
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[[Category: Leveles, I.]]
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[[Category: Lopata A]]
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[[Category: Lopata, A.]]
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[[Category: Nagy G]]
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[[Category: Nagy, G.]]
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[[Category: Takacs E]]
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[[Category: Takacs, E.]]
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[[Category: Toth J]]
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[[Category: Toth, J.]]
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[[Category: Vertessy BG]]
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[[Category: Vertessy, B G.]]
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[[Category: Hydrolase]]
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[[Category: Jelly-roll]]
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[[Category: Nucleotide metabolism]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jan 28 14:45:12 2010''
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Current revision

Structure of the mycobacterium tuberculosis DUTPase D28N mutant

PDB ID 3h6d

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