2x3b
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 2x3b is ON HOLD until Paper Publication Authors: Bogdanovic, X., Palm, G.J., Singh, R.K., Hinrichs, W. Description: AsaP1 inactive mutant E294A, an...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==AsaP1 inactive mutant E294A, an extracellular toxic zinc metalloendopeptidase== | |
| + | <StructureSection load='2x3b' size='340' side='right'caption='[[2x3b]], [[Resolution|resolution]] 2.28Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2x3b]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aeromonas_salmonicida_subsp._achromogenes Aeromonas salmonicida subsp. achromogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X3B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X3B FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.28Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x3b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x3b OCA], [https://pdbe.org/2x3b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x3b RCSB], [https://www.ebi.ac.uk/pdbsum/2x3b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x3b ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q8GMV9_AERSA Q8GMV9_AERSA] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The Gram-negative bacterium Aeromonas salmonicida is a fish pathogen for various fish species worldwide. Aeromonas salmonicida subsp. achromogenes produces the extracellular, toxic zinc endopeptidase AsaP1. Crystal structure analyses at 2.0 A resolution of two proteolytically inactive AsaP1 variants show the polypeptide folding of the protease domain and the propeptide domain. These first crystal structure analyses of a precursor of a deuterolysin-like aspzincin protease provide insights into propeptide function, and specific substrate binding. A lysine side chain of the propeptide binds in the hydrophobic S1'-pocket interacting with three carboxylate side chains. An AsaP1 variant with a lysine to alanine exchange identifies the chaperone function of the propeptide. | ||
| - | + | Structural evidence of intramolecular propeptide inhibition of the aspzincin metalloendopeptidase AsaP1.,Bogdanovic X, Palm GJ, Schwenteit J, Singh RK, Gudmundsdottir BK, Hinrichs W FEBS Lett. 2016 Aug 16. doi: 10.1002/1873-3468.12356. PMID:27528449<ref>PMID:27528449</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 2x3b" style="background-color:#fffaf0;"></div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Aeromonas salmonicida subsp. achromogenes]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Bogdanovic X]] | ||
| + | [[Category: Hinrichs W]] | ||
| + | [[Category: Palm GJ]] | ||
| + | [[Category: Singh RK]] | ||
Current revision
AsaP1 inactive mutant E294A, an extracellular toxic zinc metalloendopeptidase
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