2w9p

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{{Seed}}
 
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[[Image:2w9p.jpg|left|200px]]
 
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==Crystal Structure of Potato Multicystatin==
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The line below this paragraph, containing "STRUCTURE_2w9p", creates the "Structure Box" on the page.
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<StructureSection load='2w9p' size='340' side='right'caption='[[2w9p]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2w9p]] is a 14 chain structure with sequence from [https://en.wikipedia.org/wiki/Solanum_tuberosum Solanum tuberosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W9P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W9P FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w9p OCA], [https://pdbe.org/2w9p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w9p RCSB], [https://www.ebi.ac.uk/pdbsum/2w9p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w9p ProSAT]</span></td></tr>
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{{STRUCTURE_2w9p| PDB=2w9p | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CYTM_SOLTU CYTM_SOLTU] Probably has a role in the plant's defense system.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w9/2w9p_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2w9p ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Potato (Solanum tuberosum) multicystatin (PMC) is a crystalline Cys protease inhibitor present in the subphellogen layer of potato tubers. It consists of eight tandem domains of similar size and sequence. Our in vitro results showed that the pH/PO(4)(-)-dependent oligomeric behavior of PMC was due to its multidomain nature and was not a characteristic of the individual domains. Using a single domain of PMC, which still maintains inhibitor activity, we identified a target protein of PMC, a putative Cys protease. In addition, our crystal structure of a representative repeating unit of PMC, PMC-2, showed structural similarity to both type I and type II cystatins. The N-terminal trunk, alpha-helix, and L2 region of PMC-2 were most similar to those of type I cystatins, while the conformation of L1 more closely resembled that of type II cystatins. The structure of PMC-2 was most similar to the intensely sweet protein monellin from Dioscorephyllum cumminisii (serendipity berry), despite a low level of sequence similarity. We present a model for the possible molecular organization of the eight inhibitory domains in crystalline PMC. The unique molecular properties of the oligomeric PMC crystal are discussed in relation to its potential function in regulating the activity of proteases in potato tubers.
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===CRYSTAL STRUCTURE OF POTATO MULTICYSTATIN===
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Characterization of Solanum tuberosum multicystatin and its structural comparison with other cystatins.,Nissen MS, Kumar GN, Youn B, Knowles DB, Lam KS, Ballinger WJ, Knowles NR, Kang C Plant Cell. 2009 Mar;21(3):861-75. Epub 2009 Mar 20. PMID:19304935<ref>PMID:19304935</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19304935}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2w9p" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19304935 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19304935}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2W9P is a 14 chains structure with sequences from [http://en.wikipedia.org/wiki/Solanum_tuberosum Solanum tuberosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W9P OCA].
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==Reference==
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<ref group="xtra">PMID:19304935</ref><references group="xtra"/>
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[[Category: Solanum tuberosum]]
[[Category: Solanum tuberosum]]
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[[Category: Ballinger, W J.]]
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[[Category: Ballinger WJ]]
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[[Category: Kang, C.]]
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[[Category: Kang C]]
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[[Category: Knowles, D B.]]
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[[Category: Knowles DB]]
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[[Category: Knowles, N R.]]
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[[Category: Knowles NR]]
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[[Category: Kumar, G N.]]
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[[Category: Kumar GN]]
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[[Category: Lam, K S.]]
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[[Category: Lam KS]]
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[[Category: Nissen, M S.]]
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[[Category: Nissen MS]]
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[[Category: Youn, B.]]
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[[Category: Youn B]]
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[[Category: Hydrolase inhibitor]]
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[[Category: Protease inhibitor]]
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[[Category: Thiol protease inhibitor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 3 09:37:23 2010''
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Current revision

Crystal Structure of Potato Multicystatin

PDB ID 2w9p

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