1lwd

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[[Image:1lwd.gif|left|200px]]<br />
 
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<applet load="1lwd" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1lwd, resolution 1.85&Aring;" />
 
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'''CRYSTAL STRUCTURE OF NADP-DEPENDENT ISOCITRATE DEHYDROGENASE FROM PORCINE HEART MITOCHONDRIA'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF NADP-DEPENDENT ISOCITRATE DEHYDROGENASE FROM PORCINE HEART MITOCHONDRIA==
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The crystal structure of porcine heart mitochondrial NADP+-dependent, isocitrate dehydrogenase (IDH) complexed with Mn2+ and isocitrate was, solved to a resolution of 1.85 A. The enzyme was expressed in Escherichia, coli, purified as a fusion protein with maltose binding protein, and, cleaved with thrombin to yield homogeneous enzyme. The structure was, determined by multiwavelength anomalous diffraction phasing using selenium, substitution in the form of selenomethionine as the anomalous scatterer., The porcine NADP+-IDH enzyme is structurally compared with the previously, solved structures of IDH from E. coli and Bacillus subtilis that share 16, and 17% identity, respectively, with the mammalian enzyme. The porcine, enzyme has a protein fold similar to the bacterial IDH structures with, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?12207025 (full description)]]
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<StructureSection load='1lwd' size='340' side='right'caption='[[1lwd]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1lwd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LWD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LWD FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ICT:ISOCITRIC+ACID'>ICT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lwd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lwd OCA], [https://pdbe.org/1lwd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lwd RCSB], [https://www.ebi.ac.uk/pdbsum/1lwd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lwd ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IDHP_PIG IDHP_PIG] Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lw/1lwd_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lwd ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1LWD is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]] with MN, SO4 and ICT as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Isocitrate_dehydrogenase_(NADP(+)) Isocitrate dehydrogenase (NADP(+))]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.42 1.1.1.42]]. Structure known Active Sites: MN1 and MN2. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LWD OCA]].
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*[[Isocitrate dehydrogenase 3D structures|Isocitrate dehydrogenase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of porcine mitochondrial NADP+-dependent isocitrate dehydrogenase complexed with Mn2+ and isocitrate. Insights into the enzyme mechanism., Ceccarelli C, Grodsky NB, Ariyaratne N, Colman RF, Bahnson BJ, J Biol Chem. 2002 Nov 8;277(45):43454-62. Epub 2002 Aug 30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12207025 12207025]
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[[Category: Large Structures]]
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[[Category: Isocitrate dehydrogenase (NADP(+))]]
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[[Category: Single protein]]
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[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
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[[Category: Bahnson, B.J.]]
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[[Category: Bahnson BJ]]
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[[Category: Ceccarelli, C.]]
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[[Category: Ceccarelli C]]
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[[Category: ICT]]
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[[Category: MN]]
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[[Category: SO4]]
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[[Category: nadp]]
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[[Category: oxidoreductase]]
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[[Category: tricarboxylic acid cycle]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 10:05:41 2007''
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Current revision

CRYSTAL STRUCTURE OF NADP-DEPENDENT ISOCITRATE DEHYDROGENASE FROM PORCINE HEART MITOCHONDRIA

PDB ID 1lwd

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