1k28

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{{Seed}}
 
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[[Image:1k28.png|left|200px]]
 
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==The Structure of the Bacteriophage T4 Cell-Puncturing Device==
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The line below this paragraph, containing "STRUCTURE_1k28", creates the "Structure Box" on the page.
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<StructureSection load='1k28' size='340' side='right'caption='[[1k28]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1k28]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K28 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K28 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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{{STRUCTURE_1k28| PDB=1k28 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k28 OCA], [https://pdbe.org/1k28 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k28 RCSB], [https://www.ebi.ac.uk/pdbsum/1k28 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k28 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BP27_BPT4 BP27_BPT4] Baseplate protein that is part of the baseplate hub. Involved in the tail assembly.<ref>PMID:12837775</ref> <ref>PMID:21129200</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k2/1k28_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k28 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Bacteriophage T4 has a very efficient mechanism for infecting cells. The key component of this process is the baseplate, located at the end of the phage tail, which regulates the interaction of the tail fibres and the DNA ejection machine. A complex of gene product (gp) 5 (63K) and gp27 (44K), the central part of the baseplate, is required to penetrate the outer cell membrane of Escherichia coli and to disrupt the intermembrane peptidoglycan layer, promoting subsequent entry of phage DNA into the host. We present here a crystal structure of the (gp5-gp27)3 321K complex, determined to 2.9 A resolution and fitted into a cryo-electron microscopy map at 17 A resolution of the baseplate-tail tube assembly. The carboxy-terminal domain of gp5 is a triple-stranded beta-helix that forms an equilateral triangular prism, which acts as a membrane-puncturing needle. The middle lysozyme domain of gp5, situated on the periphery of the prism, serves to digest the peptidoglycan layer. The amino-terminal, antiparallel beta-barrel domain of gp5 is inserted into a cylinder formed by three gp27 monomers, which may serve as a channel for DNA ejection.
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===The Structure of the Bacteriophage T4 Cell-Puncturing Device===
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Structure of the cell-puncturing device of bacteriophage T4.,Kanamaru S, Leiman PG, Kostyuchenko VA, Chipman PR, Mesyanzhinov VV, Arisaka F, Rossmann MG Nature. 2002 Jan 31;415(6871):553-7. PMID:11823865<ref>PMID:11823865</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1k28" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_11823865}}, adds the Publication Abstract to the page
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*[[Lysozyme 3D structures|Lysozyme 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 11823865 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_11823865}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Escherichia virus T4]]
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1K28 is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K28 OCA].
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[[Category: Large Structures]]
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[[Category: Arisaka F]]
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==Reference==
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[[Category: Chipman PR]]
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<ref group="xtra">PMID:11823865</ref><references group="xtra"/>
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[[Category: Kanamaru S]]
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[[Category: Enterobacteria phage t4]]
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[[Category: Kostyuchenko VA]]
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[[Category: Lysozyme]]
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[[Category: Leiman PG]]
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[[Category: Arisaka, F.]]
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[[Category: Mesyanzhinov VV]]
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[[Category: Chipman, P R.]]
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[[Category: Rossmann MG]]
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[[Category: Kanamaru, S.]]
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[[Category: Kostyuchenko, V A.]]
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[[Category: Leiman, P G.]]
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[[Category: Mesyanzhinov, V V.]]
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[[Category: Rossmann, M G.]]
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[[Category: Gp27-gp5*-gp5c]]
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[[Category: Hub]]
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[[Category: Hydrolase/structural protein complex]]
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[[Category: Ob fold]]
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[[Category: Pseudohexamer]]
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[[Category: T4 tail lysozyme]]
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[[Category: Triple-stranded beta-helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 7 22:03:47 2010''
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Current revision

The Structure of the Bacteriophage T4 Cell-Puncturing Device

PDB ID 1k28

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